Document Detail


Memory B cells producing somatically mutated antiphospholipid antibodies are present in healthy individuals.
MedLine Citation:
PMID:  12791657     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antiphospholipid antibodies (aPLs) are associated with thrombosis and recurrent abortions during autoimmune pathologies, but they are also produced in healthy individuals and during infectious diseases. To analyze the possible links between physiologic and pathologic aPLs, it is of importance to characterize normal aPL production. We took advantage of the known tropism of Epstein-Barr virus (EBV) for B cells in general, and memory B cells in particular, during primary infectious mononucleosis (IMN) in 3 patients to get access to anticardiolipin (aCL)-producing B cells. Flow cytometry analysis of these cells showed that, depending on the patient, 10% to 60% of immunoglobulin M (IgM) aCL-producing B cells express the CD27 marker of memory B cells. Single cell sorting of aCL B cells, followed by single cell reverse transcription-polymerase chain reaction (RT-PCR) amplification of their immunoglobulin variable region genes, showed that some of these cells produce mutated forms of aCL antibodies, confirming their memory B-cell origin. Considering that, during primary IMN, EBV infects and expands already pre-existing memory B cells, we conclude that healthy individuals have a discrete pool of aCL memory cells able to produce mutated forms of antibodies. The implications of this new information are discussed in light of different hypotheses regarding the origin of aCL.
Authors:
Patricia Lieby; Anne Soley; Anne-Marie Knapp; Martine Cerutti; Jean-Marie Freyssinet; Jean-Louis Pasquali; Thierry Martin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-06-05
Journal Detail:
Title:  Blood     Volume:  102     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-09-23     Completed Date:  2003-10-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2459-65     Citation Subset:  AIM; IM    
Affiliation:
Laboratoire d'Immunopathologie, Institut National de la Santé et de la Recherche Médicale EMI 0222, Strasbourg, France.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Antibodies, Anticardiolipin / genetics*,  immunology
Antibodies, Monoclonal / immunology
B-Lymphocytes / immunology*
Female
Flow Cytometry
Gene Rearrangement, B-Lymphocyte / immunology
Humans
Immunologic Memory / genetics*,  immunology
Immunophenotyping
Infectious Mononucleosis / immunology
Male
Chemical
Reg. No./Substance:
0/Antibodies, Anticardiolipin; 0/Antibodies, Monoclonal

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