| Memory B cells producing somatically mutated antiphospholipid antibodies are present in healthy individuals. | |
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MedLine Citation:
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PMID: 12791657 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Antiphospholipid antibodies (aPLs) are associated with thrombosis and recurrent abortions during autoimmune pathologies, but they are also produced in healthy individuals and during infectious diseases. To analyze the possible links between physiologic and pathologic aPLs, it is of importance to characterize normal aPL production. We took advantage of the known tropism of Epstein-Barr virus (EBV) for B cells in general, and memory B cells in particular, during primary infectious mononucleosis (IMN) in 3 patients to get access to anticardiolipin (aCL)-producing B cells. Flow cytometry analysis of these cells showed that, depending on the patient, 10% to 60% of immunoglobulin M (IgM) aCL-producing B cells express the CD27 marker of memory B cells. Single cell sorting of aCL B cells, followed by single cell reverse transcription-polymerase chain reaction (RT-PCR) amplification of their immunoglobulin variable region genes, showed that some of these cells produce mutated forms of aCL antibodies, confirming their memory B-cell origin. Considering that, during primary IMN, EBV infects and expands already pre-existing memory B cells, we conclude that healthy individuals have a discrete pool of aCL memory cells able to produce mutated forms of antibodies. The implications of this new information are discussed in light of different hypotheses regarding the origin of aCL. |
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Authors:
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Patricia Lieby; Anne Soley; Anne-Marie Knapp; Martine Cerutti; Jean-Marie Freyssinet; Jean-Louis Pasquali; Thierry Martin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-06-05 |
Journal Detail:
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Title: Blood Volume: 102 ISSN: 0006-4971 ISO Abbreviation: Blood Publication Date: 2003 Oct |
Date Detail:
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Created Date: 2003-09-23 Completed Date: 2003-10-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: United States |
Other Details:
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Languages: eng Pagination: 2459-65 Citation Subset: AIM; IM |
Affiliation:
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Laboratoire d'Immunopathologie, Institut National de la Santé et de la Recherche Médicale EMI 0222, Strasbourg, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Antibodies, Anticardiolipin / genetics*, immunology Antibodies, Monoclonal / immunology B-Lymphocytes / immunology* Female Flow Cytometry Gene Rearrangement, B-Lymphocyte / immunology Humans Immunologic Memory / genetics*, immunology Immunophenotyping Infectious Mononucleosis / immunology Male |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Anticardiolipin; 0/Antibodies, Monoclonal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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