Document Detail


Membrane transporters and cytoplasmatic pH regulation on bovine Sertoli cells.
MedLine Citation:
PMID:  19050954     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sertoli cells are responsible for regulating a wide range of processes that lead to the differentiation of male germ cells into spermatozoa. Cytoplasmic pH (pHi) has been shown to be an important parameter in cell physiology, regulating namely cell metabolism and differentiation. However, membrane transport mechanisms involved in pHi regulation mechanisms of Sertoli cells have not yet been elucidated. In this work, pHi was determined using the pH-sensitive fluorescent probe 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF). Addition of weak acids resulted in rapid acidification of the intracellular milieu. Sertoli cells then recovered pHi by a mechanism that was shown to be sensitive to external Na+. pHi recovery was also greatly reduced in the presence of 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) and amiloride. These results point toward the action of an Na+-driven HCO3-/Cl- exchanger and/or an Na+/HCO3- cotransporter and the action of the Na+/H+ exchanger on pHi regulation in the experimental conditions used. pHi recovery was only slightly affected by ouabain, suggesting that the inhibition of Na+/K+-ATPase affects recovery indirectly, possibly via the shift on the Na+ gradient. On the other hand, recovery from the acid load was independent of the presence of concanamycin A, a specific inhibitor of the V-type ATPases, suggesting that these pumps do not have a relevant action on pHi regulation in bovine Sertoli cells.
Authors:
P F Oliveira; M Sousa; A Barros; T Moura; A Rebelo da Costa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-03
Journal Detail:
Title:  The Journal of membrane biology     Volume:  227     ISSN:  0022-2631     ISO Abbreviation:  J. Membr. Biol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-31     Completed Date:  2009-03-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0211301     Medline TA:  J Membr Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  49-55     Citation Subset:  IM    
Affiliation:
UMIB, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto, L. Prof. Abel Salazar, 2, 4099-003, Porto, Portugal. transp.bio@mail.icav.up.pt
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MeSH Terms
Descriptor/Qualifier:
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / metabolism
Acids / metabolism
Amiloride / metabolism
Animals
Bicarbonates / metabolism
Cattle
Cells, Cultured
Chlorides / metabolism
Cytoplasm / metabolism*
Fluoresceins / diagnostic use
Fluorescent Dyes / diagnostic use
Hydrogen-Ion Concentration
Male
Membrane Transport Proteins / chemistry,  metabolism*
Sertoli Cells / metabolism*
Sodium-Potassium-Exchanging ATPase / metabolism
Chemical
Reg. No./Substance:
0/Acids; 0/Bicarbonates; 0/Chlorides; 0/Fluoresceins; 0/Fluorescent Dyes; 0/Membrane Transport Proteins; 2609-46-3/Amiloride; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 85138-49-4/2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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