| Membrane topology of the ATP-binding cassette transporter associated with antigen presentation (Tap1) expressed in Escherichia coli. | |
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MedLine Citation:
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PMID: 9111005 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ATP-binding cassette transporters associated with antigen presentation (Tap1 and Tap2) mediate the transport of peptide fragments across the endoplasmic reticulum membrane of mammalian cells. Tap1 and Tap2 are closely related to one another and are believed to function as a heterodimer. Each protein possesses a hydrophobic domain predicted to span the membrane multiple times and a highly conserved nucleotide-binding domain. We have assessed the transmembrane topology of Tap1 by expressing a series of fusions to a reporter protein, the mature form of beta-lactamase in Escherichia coli. From these data a topological model can be derived in which Tap1 spans the membrane eight times, with several large loops exposed in the lumen of the endoplasmic reticulum and with both the N and C termini (including the nucelotide-binding domain) residing in the cytoplasm. |
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Authors:
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U Gileadi; C F Higgins |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 272 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1997 Apr |
Date Detail:
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Created Date: 1997-05-21 Completed Date: 1997-05-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 11103-8 Citation Subset: IM |
Affiliation:
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Nuffield Department of Clinical Biochemistry and Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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ATP-Binding Cassette Transporters
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chemistry*,
genetics Amino Acid Sequence Antigen Presentation* Carrier Proteins / chemistry Cloning, Molecular Escherichia coli / genetics Escherichia coli Proteins* Genes, Reporter Humans Molecular Sequence Data Monosaccharide Transport Proteins* Protein Conformation Recombinant Fusion Proteins / chemistry Sequence Homology, Amino Acid beta-Lactamases / chemistry, genetics |
| Chemical | |
Reg. No./Substance:
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0/ATP-Binding Cassette Transporters; 0/Carrier Proteins; 0/Escherichia coli Proteins; 0/Monosaccharide Transport Proteins; 0/Recombinant Fusion Proteins; 0/TAP1 protein, human; 0/maltose transport system, E coli; 0/maltose-binding protein; 145892-13-3/TAP2 protein, human; EC 3.5.2.6/beta-Lactamases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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