Document Detail

Membrane topology of the ATP-binding cassette transporter associated with antigen presentation (Tap1) expressed in Escherichia coli.
MedLine Citation:
PMID:  9111005     Owner:  NLM     Status:  MEDLINE    
The ATP-binding cassette transporters associated with antigen presentation (Tap1 and Tap2) mediate the transport of peptide fragments across the endoplasmic reticulum membrane of mammalian cells. Tap1 and Tap2 are closely related to one another and are believed to function as a heterodimer. Each protein possesses a hydrophobic domain predicted to span the membrane multiple times and a highly conserved nucleotide-binding domain. We have assessed the transmembrane topology of Tap1 by expressing a series of fusions to a reporter protein, the mature form of beta-lactamase in Escherichia coli. From these data a topological model can be derived in which Tap1 spans the membrane eight times, with several large loops exposed in the lumen of the endoplasmic reticulum and with both the N and C termini (including the nucelotide-binding domain) residing in the cytoplasm.
U Gileadi; C F Higgins
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  272     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-05-21     Completed Date:  1997-05-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  11103-8     Citation Subset:  IM    
Nuffield Department of Clinical Biochemistry and Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom.
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MeSH Terms
ATP-Binding Cassette Transporters / chemistry*,  genetics
Amino Acid Sequence
Antigen Presentation*
Carrier Proteins / chemistry
Cloning, Molecular
Escherichia coli / genetics
Escherichia coli Proteins*
Genes, Reporter
Molecular Sequence Data
Monosaccharide Transport Proteins*
Protein Conformation
Recombinant Fusion Proteins / chemistry
Sequence Homology, Amino Acid
beta-Lactamases / chemistry,  genetics
Reg. No./Substance:
0/ATP-Binding Cassette Transporters; 0/Carrier Proteins; 0/Escherichia coli Proteins; 0/Monosaccharide Transport Proteins; 0/Recombinant Fusion Proteins; 0/TAP1 protein, human; 0/maltose transport system, E coli; 0/maltose-binding protein; 145892-13-3/TAP2 protein, human; EC

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