Document Detail


Membrane structure-function relationships in cell-mediated cytolysis. I. Effect of exogenously incorporated fatty acids on effector cell function in cell-mediated cytolysis.
MedLine Citation:
PMID:  6446577     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fatty acid composition of the membrane phospholipids of CTL memory cells restimulated with Con A can be altered significantly when selected exogenous fatty acids are present during the restimulation culture period. Such alterations also lead to significant changes in the subsequent cytolytic capacity of the regenerated CTL. Unsaturated fatty acids increase cytotoxicity, whereas saturated fatty acids lead to decreased cytotoxic function. The kinetics of the fatty acid effect on CTL function is the same as the kinetics of fatty acid incorporation into membrane phospholipids, suggesting that alteration of cytotoxicity results from altering membrane phospholipid fatty acid composition. The initial studies reported here suggest that this effect is mediated at the level of individual CTL, rather than through an alteration of CTL frequency in effector cell populations. Moreover, it seems unlikely that the fatty acids mediate their effect by altering membrane fluidity.
Authors:
R Gill; W Clark
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  125     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1980 Aug 
Date Detail:
Created Date:  1980-09-26     Completed Date:  1980-09-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  689-95     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism
Animals
Binding Sites
Cell Membrane / immunology
Cells, Cultured
Concanavalin A / pharmacology
Cytotoxicity, Immunologic*
Dose-Response Relationship, Immunologic
Fatty Acids / pharmacology*
Female
Immunologic Memory
Kinetics
Lymphocyte Culture Test, Mixed
Mice
Mice, Inbred C3H
Mice, Inbred DBA
Phospholipids / immunology*
Thymidine / metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Fatty Acids; 0/Phospholipids; 11028-71-0/Concanavalin A; 50-89-5/Thymidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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