Document Detail


Membrane segregation in atrioventricular nodal myocytes of the golden hamster (Mesocricetus auratus). A cytochemical study using filipin and tomatine.
MedLine Citation:
PMID:  2606769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The membrane systems of hamster general atrial and ventricular myocytes were studied and their reactivity with the sterol probes, filipin and tomatine, compared with that of atrioventricular nodal myocytes. The sarcolemma of atrial and atrioventricular nodal myocytes was considerably more reactive to sterol probes than the sarcolemma of general ventricular myocytes. Atrial myocyte sarcolemma was also more reactive than previously reported in rat atrial myocytes. Particle-free regions of annular nexuses were consistently unreactive with all combinations of sterol probes whereas much smaller particle-free regions of macular nexuses and desmosomes were reactive with the same probes. Intracellular membrane systems of all three types of cardiac myocyte reacted in a similar manner with sterol probes. These results indicate variation in comparable sarcolemma composition of cardiac myocytes between the rat and hamster and between cardiac regions within the same species.
Authors:
J N Skepper
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of anatomy     Volume:  163     ISSN:  0021-8782     ISO Abbreviation:  J. Anat.     Publication Date:  1989 Apr 
Date Detail:
Created Date:  1990-02-22     Completed Date:  1990-02-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0137162     Medline TA:  J Anat     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  143-54     Citation Subset:  IM    
Affiliation:
Department of Anatomy, University of Cambridge.
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MeSH Terms
Descriptor/Qualifier:
Animals
Atrioventricular Node / cytology,  metabolism,  ultrastructure
Cell Membrane / drug effects,  metabolism,  ultrastructure
Cricetinae / anatomy & histology*
Female
Filipin / diagnostic use,  pharmacology
Freeze Fracturing
Histocytochemistry / methods
Mesocricetus / anatomy & histology*
Microscopy, Electron
Myocardium / cytology*,  metabolism,  ultrastructure
Sterols / metabolism
Tomatine / diagnostic use,  pharmacology
Chemical
Reg. No./Substance:
0/Sterols; 17406-45-0/Tomatine; 480-49-9/Filipin
Comments/Corrections

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