| Membrane-rich milk fat diet provides protection against gastrointestinal leakiness in mice treated with lipopolysaccharide. | |
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MedLine Citation:
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PMID: 21524510 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Milk fat globule membrane is a protein-lipid complex that may strengthen the gut barrier. The main objective of this study was to assess the ability of a membrane-rich milk fat diet to promote the integrity of the gut barrier and to decrease systemic inflammation in lipopolysaccharide (LPS)-challenged mice. Animals were randomly assigned to one of 2 American Institute of Nutrition (AIN)-76A formulations differing only in fat source: control diet (corn oil) and milk fat diet (anhydrous milk fat with 10% milk fat globule membrane). Each diet contained 12% calories from fat. Mice were fed diets for 5 wk, then injected with vehicle or LPS (10mg/kg of BW) and gavaged with dextran-fluorescein to assess gut barrier integrity. Serum was assayed for fluorescence 24h after gavage, and 16 serum cytokines were measured to assess the inflammatory response. Gut permeability was 1.8-fold higher in LPS-challenged mice fed the control diet compared with the milk fat diet. Furthermore, mice fed the milk fat diet and injected with LPS had lower serum levels of IL-6, IL-10, IL-17, monocyte chemotactic protein (MCP)-1, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and IL-3 compared with LPS-injected mice fed the control diet. The results indicate that the membrane-rich milk fat diet decreases the inflammatory response to a systemic LPS challenge compared with corn oil, and the effect coincides with decreased gut permeability. |
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Authors:
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D R Snow; R E Ward; A Olsen; R Jimenez-Flores; K J Hintze |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of dairy science Volume: 94 ISSN: 1525-3198 ISO Abbreviation: J. Dairy Sci. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: United States |
Other Details:
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Languages: eng Pagination: 2201-12 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Nutrition, Dietetics and Food Sciences, Utah State University, Logan 84322. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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