Document Detail


Membrane proteomics of phagosomes suggests a connection to autophagy.
MedLine Citation:
PMID:  18971338     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phagocytosis is the central process by which macrophage cells internalize and eliminate infectious microbes as well as apoptotic cells. During maturation, phagosomes containing engulfed particles fuse with various endosomal compartments through the action of regulatory molecules on the phagosomal membrane. In this study, we performed a proteomic analysis of the membrane fraction from latex bead-containing (LBC) phagosomes isolated from macrophages. The profile, which comprised 546 proteins, suggests diverse functions of the phagosome and potential connections to secretory processes, toll-like receptor signaling, and autophagy. Many identified proteins were not previously known to reside in the phagosome. We characterized several proteins in LBC phagosomes that change in abundance on induction of autophagy, a process that has been previously implicated in the host defense against microbial pathogens. These observations suggest crosstalk between autophagy and phagocytosis that may be relevant to the innate immune response of macrophages.
Authors:
Wenqing Shui; Leslie Sheu; Jun Liu; Brian Smart; Christopher J Petzold; Tsung-Yen Hsieh; Austin Pitcher; Jay D Keasling; Carolyn R Bertozzi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-10-29
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  105     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-05     Completed Date:  2008-12-22     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  16952-7     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autophagy*
Cell Membrane / metabolism
Macrophages / immunology*,  metabolism
Membrane Proteins / analysis,  metabolism*
Mice
Phagocytosis / immunology,  physiology
Phagosomes / immunology,  metabolism*
Proteome / analysis,  metabolism
Proteomics / methods
Grant Support
ID/Acronym/Agency:
AI51622/AI/NIAID NIH HHS; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Membrane Proteins; 0/Proteome
Comments/Corrections

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