Document Detail

Membrane perturbation by mastoparan 7 elicits a broad alteration in lipid composition of L1210 cells.
MedLine Citation:
PMID:  10760465     Owner:  NLM     Status:  MEDLINE    
Mastoparan 7 (Mas-7), an amphiphilic peptide possessing membrane perturbing activity, has been known to selectively stimulate some lipases. To examine changes in the lipid composition induced by Mas-7, we carried out systemic lipid analysis of L1210 cells after Mas-7 treatment. The total lipid was determined by HPLC, gas-liquid chromatography, and electrospray ionization mass spectrometry in conjunction with differential radiolabelling with [(32)P]orthophosphate, [(3)H]myristic acid, and [(3)H]arachidonic acid. The lipid analysis revealed multiple changes in more than 10 lipid classes. Free fatty acids (FFAs) and phosphatidylethanol (PEt), the phospholipase D product in the presence of ethanol, were increased significantly and phosphatidylcholine (PC) was decreased. Digitonin, a membrane permeabilizing reagent, similarly affected the lipid composition of L1210. The FFA released showed a very broad distribution of saturated, monounsaturated, and polyunsaturated fatty acids, implying that phospholipase A(2) alone could not account for all of the FFAs released. By comparing the molecular species of PEt with those of endogenous PC, we showed that phospholipase D in L1210 cells appeared to act selectively on diacyl-PC. The perturbation-induced alterations in the lipid composition brought about by Mas-7 might play a crucial role in the physiology of the affected cells.
H S Park; S Y Lee; Y H Kim; J Y Kim; S J Lee; M Choi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1484     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-06-23     Completed Date:  2000-06-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  151-62     Citation Subset:  IM    
Department of Chemistry and Center for Molecular Catalysis, Seoul National University, Seoul, South Korea.
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MeSH Terms
Cell Membrane / drug effects*
Chromatography, High Pressure Liquid
Digitonin / pharmacology
Fatty Acids, Nonesterified / analysis
Glycerophospholipids / analysis
Leukemia L1210
Mass Spectrometry
Membrane Lipids / chemistry*
Peptides / pharmacology*
Phosphatidylcholines / analysis
Phospholipids / analysis*
Phosphorus Radioisotopes
Tumor Cells, Cultured
Wasp Venoms / pharmacology
Reg. No./Substance:
0/Fatty Acids, Nonesterified; 0/Glycerophospholipids; 0/Mas7 protein, synthetic; 0/Membrane Lipids; 0/Peptides; 0/Phosphatidylcholines; 0/Phospholipids; 0/Phosphorus Radioisotopes; 0/Wasp Venoms; 0/phosphatidylethanol; 10028-17-8/Tritium; 11024-24-1/Digitonin; 72093-21-1/mastoparan

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