| Membrane glycoprotein changes during the senescence of normal human diploid fibroblasts in culture. | |
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MedLine Citation:
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PMID: 4021559 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The plasma membrane is intimately involved in a variety of cellular phenomena and may play an important role in the aging of human cells in culture. Significant differences in human diploid fibroblast surface glycoproteins were observed with in vitro aging. Senescent cells bound more concanavalin A (Con A) than young cells and exhibited two distinct classes of binding sites. Cell surfaces of senescent cells incorporated less labelled mannose and more labelled fucose and glucosamine than young cell surfaces. Membranes prepared from older cells were also less effective than membrane preparations from young cells in incorporating mannose from GDP-mannose into a group of oligolipid intermediates, required for the synthesis of glycoproteins with asparagine-linked oligosaccharides. These results demonstrate novel quantitative changes in membrane structure and lectin reactivity in aging human diploid fibroblasts, which must reflect the fundamental physiological modifications in the cell that occur during senescence. |
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Authors:
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J A Blondal; J E Dick; J A Wright |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Mechanisms of ageing and development Volume: 30 ISSN: 0047-6374 ISO Abbreviation: Mech. Ageing Dev. Publication Date: 1985 May |
Date Detail:
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Created Date: 1985-09-24 Completed Date: 1985-09-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0347227 Medline TA: Mech Ageing Dev Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 273-83 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Membrane
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enzymology,
metabolism Cell Survival* Cells, Cultured Concanavalin A / metabolism Fibroblasts / enzymology, metabolism Glycoproteins / metabolism* Humans Mannosyltransferases / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Glycoproteins; 11028-71-0/Concanavalin A; EC 2.4.1.-/Mannosyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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