Document Detail

Membrane glycoprotein changes during the senescence of normal human diploid fibroblasts in culture.
MedLine Citation:
PMID:  4021559     Owner:  NLM     Status:  MEDLINE    
The plasma membrane is intimately involved in a variety of cellular phenomena and may play an important role in the aging of human cells in culture. Significant differences in human diploid fibroblast surface glycoproteins were observed with in vitro aging. Senescent cells bound more concanavalin A (Con A) than young cells and exhibited two distinct classes of binding sites. Cell surfaces of senescent cells incorporated less labelled mannose and more labelled fucose and glucosamine than young cell surfaces. Membranes prepared from older cells were also less effective than membrane preparations from young cells in incorporating mannose from GDP-mannose into a group of oligolipid intermediates, required for the synthesis of glycoproteins with asparagine-linked oligosaccharides. These results demonstrate novel quantitative changes in membrane structure and lectin reactivity in aging human diploid fibroblasts, which must reflect the fundamental physiological modifications in the cell that occur during senescence.
J A Blondal; J E Dick; J A Wright
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mechanisms of ageing and development     Volume:  30     ISSN:  0047-6374     ISO Abbreviation:  Mech. Ageing Dev.     Publication Date:  1985 May 
Date Detail:
Created Date:  1985-09-24     Completed Date:  1985-09-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0347227     Medline TA:  Mech Ageing Dev     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  273-83     Citation Subset:  IM    
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MeSH Terms
Cell Membrane / enzymology,  metabolism
Cell Survival*
Cells, Cultured
Concanavalin A / metabolism
Fibroblasts / enzymology,  metabolism
Glycoproteins / metabolism*
Mannosyltransferases / metabolism
Reg. No./Substance:
0/Glycoproteins; 11028-71-0/Concanavalin A; EC 2.4.1.-/Mannosyltransferases

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