| Membrane fusion between liposomes composed of acidic phospholipids and neutral phospholipids induced by melittin: a differential scanning calorimetric study. | |
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MedLine Citation:
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PMID: 11530015 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Melittin-induced membrane fusion between neutral and acidic phospholipids was examined in liposome systems with a high-sensitivity differential scanning calorimeter. Membrane fusion could be detected by calorimetric measurement by observing thermograms of mixed liposomal lipids. The roles of hydrophobic and electrostatic interactions were investigated in membrane fusion induced by melittin. Melittin, a bee venom peptide, is composed of a hydrophobic region including hydrophobic amino acids and a positively charged region including basic amino acids. When phosphatidylcholine liposomes were prepared in the presence of melittin, reductions in the phase transition enthalpies were observed in the following order; dimyristoylphosphatidylcholine (DMPC) > dipalmitoylphosphatidylcholine (DPPC) > distearoylphosphatidylcholine (DSPC) > dielaidoylphosphatidylcholine (DEPC). The plase transition enthalpy of an acidic phospholipid, dipalmitoylphosphatidylserine (DPPS), was raised by melittin at low concentrations, then reduced at higher concentrations. DPPC liposomes prepared in melittin solution were fused with DPPS liposomes when the liposomal dispersions were mixed and incubated. Similar fusion was observed between dipalmitoylphosphatidylcholine and dimyristoylphosphatidic acid (DMPA) liposomes. These results indicate that a peptide including hydrophobic and basic regions can mediate membrane fusion between neutral and acidic liposomes by hydrophobic and electrostatic interactions. |
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Authors:
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Y Higashino; A Matsui; K Ohki |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biochemistry Volume: 130 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 2001 Sep |
Date Detail:
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Created Date: 2001-08-31 Completed Date: 2001-12-20 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 393-7 Citation Subset: IM |
Affiliation:
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Department of Physics, Graduate School of Science, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Calorimetry, Differential Scanning Glycerophospholipids / metabolism Liposomes / chemistry, metabolism* Melitten / metabolism*, pharmacology Membrane Fusion / drug effects, physiology* Membrane Lipids / chemistry, metabolism Phosphatidylcholines / metabolism Phosphatidylserines / metabolism Phospholipids / metabolism* Static Electricity Thermodynamics |
| Chemical | |
Reg. No./Substance:
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0/Glycerophospholipids; 0/Liposomes; 0/Membrane Lipids; 0/Phosphatidylcholines; 0/Phosphatidylserines; 0/Phospholipids; 20449-79-0/Melitten; 30170-00-4/dimyristoylphosphatidic acid; 3036-82-6/dipalmitoylphosphatidylserine |
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