Document Detail

Membrane dynamics in the action of dibutyryl adenosine 3':5'-cyclic monophosphate and testosterone on mammalian cells.
MedLine Citation:
PMID:  4340166     Owner:  NLM     Status:  MEDLINE    
The first manifestation of reverse transformation, in which a permanent epithelial-like cell is reversibly transformed into a fibroblast-like cell by dibutyryl adenosine cyclic 3':5'-monophosphate, is the disappearance of a set of violently extending and retracting knobbed structures on the cell membrane with consequent tranquilization of the membrane. The cell then elongates to assume a fibroblast-like morphology. Permanently fibroblast-like cells, which have smooth membranes, can be changed into knobbed epithelial-like cells by the addition of either colcemid or cytochalasin B, agents that disorganize one or the other component, respectively, of the microtubular-microfibrillar system. Permanently smoothmembraned, epithelial-like cells also become knobbed by such treatment. All of these knobbed epithelial-like cells can be converted into smooth-membraned fibroblast-like cells by dibutyryl cAMP alone or with the aid of supporting molecules like testosterone. It is proposed that these are general phenomena and that the knobbed epithelial-like forms and the more highly differentiated smooth-membraned fibroblast-like cells represent extreme situations with respect to the degree of organization of the microtubular-microfibrillar system inside the cell.
T T Puck; C A Waldren; A W Hsie
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  69     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1972 Jul 
Date Detail:
Created Date:  1972-09-22     Completed Date:  1972-09-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1943-7     Citation Subset:  IM    
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MeSH Terms
Biological Products / pharmacology
Butyrates / pharmacology
Cell Line
Cell Membrane / drug effects*
Cyclic AMP / pharmacology*
Demecolcine / pharmacology
Drug Synergism
Hela Cells / cytology,  drug effects*
Indoles / pharmacology
Lung / cytology,  drug effects*
Microtubules / drug effects
Motion Pictures as Topic
Ovary / cytology,  drug effects*
Testosterone / pharmacology*
Vinblastine / pharmacology
Reg. No./Substance:
0/Biological Products; 0/Butyrates; 0/Indoles; 477-30-5/Demecolcine; 58-22-0/Testosterone; 60-92-4/Cyclic AMP; 865-21-4/Vinblastine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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