Document Detail


Membrane depolarization of human B cells follows stimulation by either anti-mu or B-cell growth factor, but only anti-mu causes cell volume changes.
MedLine Citation:
PMID:  3131276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human peripheral blood B cells were separated from monocytes and T cells, depleted of null cells by an anti-Leu 9 rosetting technique, and fractionated on discontinuous Percoll gradients to yield a highly purified, small, dense B-cell population. These cells responded to F(ab')2 goat anti-mu at 10 and 100 micrograms/ml with membrane depolarization (measured by immunofluorescence with 3,3'-dipentyloxacarbocyanine dye) at 1 h, cell volume enlargement by 48 h, and modest thymidine incorporation by 72 h. They also responded to the 12-kd human B-cell growth factor of Maizel with membrane depolarization, but not with cell volume increase. F(ab')2 anti-mu and B-cell growth factor together induced greater depolarization than was seen with either alone, but there was no synergy. The cell volume increase seen with F(ab')2 anti-mu was not increased by B-cell growth factor. Comparison of data analysis methods showed that mean fluorescence intensity most readily detected significant depolarization. We conclude that in human B cells: (1) depolarization may be a "general response" to a variety of membrane stimuli, because F(ab')2 anti-mu and B-cell growth factor acting through different receptors both induce it, and (2) depolarization does not inevitably lead to cell volume increase.
Authors:
W M Yokoyama; M M Chien; S E Engardt; S W Aguiar; R F Ashman
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Human immunology     Volume:  21     ISSN:  0198-8859     ISO Abbreviation:  Hum. Immunol.     Publication Date:  1988 Mar 
Date Detail:
Created Date:  1988-07-08     Completed Date:  1988-07-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8010936     Medline TA:  Hum Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  155-64     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, University of Iowa College of Medicine, Iowa City.
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MeSH Terms
Descriptor/Qualifier:
Antigen-Antibody Reactions
B-Lymphocytes / immunology*
Carbocyanines
Cell Separation
Flow Cytometry
Fluorescent Dyes
Humans
Immunoglobulin mu-Chains / immunology*
Interleukin-4
Interleukins / immunology*
Lymphocyte Activation*
Membrane Potentials
Grant Support
ID/Acronym/Agency:
GM36261/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Carbocyanines; 0/Fluorescent Dyes; 0/Immunoglobulin mu-Chains; 0/Interleukins; 207137-56-2/Interleukin-4; 60031-82-5/3,3'-dipentyl-2,2'-oxacarbocyanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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