Document Detail

Membrane composition influences the topology bias of bacterial integral membrane proteins.
MedLine Citation:
PMID:  22982496     Owner:  NLM     Status:  Publisher    
Small multidrug resistance (SMR) protein family members confer bacterial resistance to toxic antiseptics and are believed to function as dual topology oligomers. If dual topology is essential for SMR activity, then the topology bias should change as bacterial membrane lipid compositions alter to maintain a "neutral" topology bias. To test this hypothesis, a bioinformatic analysis of bacterial SMR protein sequences was performed to determine a membrane protein topology based on charged amino acid residues within loops, and termini regions according to the positive inside rule. Three bacterial lipid membrane parameters were examined, providing the proportion of polar lipid head group charges at the membrane surface (PLH), the relative hydrophobic fatty acid length (FAL), and the proportion of fatty acid unsaturation (FAU). Our analysis indicates that individual SMR pairs, and to a lesser extent SMR singleton topology biases, are significantly correlated to increasing PLH, FAL and FAU differences validating the hypothesis. Correlations between the topology biases of SMR proteins identified in Gram+compared to Gram- species and each lipid parameter demonstrated a linear inverse relationship.
Denice C Bay; Raymond J Turner
Related Documents :
25436476 - A high-temperature dielectric process as a probe of large-scale silica filler structure...
22932996 - Membrane trafficking in podocyte health and disease.
23085196 - Cytochrome c produces pores in cardiolipin-containing planar bilayer lipid membranes in...
24174146 - Membrane structure: morphological and chemical alterations in phospholipase-c-treated m...
16542086 - Positronium annihilation lifetimes and dielectric spectroscopy studies on diethyl phtha...
17614636 - Instrumented tracer for lagrangian measurements in rayleigh-bénard convection.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-13
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  -     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Rapid Bactericidal Action of Alpha-mangostin Against MRSA as an Outcome of Membrane Targeting.
Next Document:  Effect of Azadinium spinosum on the feeding behaviour and azaspiracid accumulation of Mytilus edulis...