Document Detail


Membrane-bound virions of coxsackievirus B4: cellular localization, analysis of the genomic RNA, genome-linked protein, and effect on host macromolecular synthesis.
MedLine Citation:
PMID:  2985028     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hela cells infected with several group B coxsackieviruses contain, in addition to standard virions, a population of virus-specific ribonucleoprotein particles which we (5) designated membrane-bound virions (MBV). MBVs differ from standard virions in buoyant density, yield, appearance, protein composition and infectivity. Here we present several new features of MBVs of coxsackievirus B4. The MBVs are lighter (rho about 1.30) and are localized in rough membranes, intermixed with virions. They contain 35S virion RNA covalently linked with a small protein, VPg. The VPg contain two proteins of different charge. MBV VPg is considerably smaller than the 5300-dalton virion VPg. MBV RNA is homologous to the base sequence present in B4 virus double-stranded RNA. The T1 oligonucleotide fingerprint of MBV RNA is distinguishable from that of virion RNA by one oligonucleotide. Several oligonucleotides of virion RNA appear to occur in submolar quantities in MBV RNA. MBVs are 75 to greater than 200 times less infective; they inhibit host cell macromolecular synthesis less efficiently than virions. In coinfected cells, the extent of inhibition of host synthesis is less severe than in cells infected with virions alone, which suggest interference by MBV particles.
Authors:
N K Chatterjee; C Nejman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of virology     Volume:  84     ISSN:  0304-8608     ISO Abbreviation:  Arch. Virol.     Publication Date:  1985  
Date Detail:
Created Date:  1985-05-15     Completed Date:  1985-05-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506870     Medline TA:  Arch Virol     Country:  AUSTRIA    
Other Details:
Languages:  eng     Pagination:  105-18     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Compartmentation
Coxsackievirus Infections / microbiology,  physiopathology*
Cytoplasm / microbiology
Enterovirus B, Human / analysis*,  genetics,  physiology
Female
Hela Cells
Humans
Intracellular Membranes / ultrastructure
Protein Biosynthesis
RNA, Viral / analysis
Viral Proteins / analysis
Virion / analysis
Chemical
Reg. No./Substance:
0/RNA, Viral; 0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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