| Membrane-bound pyrroloquinoline quinone-dependent dehydrogenase in Gluconobacter oxydans M5, responsible for production of 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose. | |
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MedLine Citation:
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PMID: 18502922 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A membrane-bound protein purified from Gluconobacter oxydans M5 was confirmed to be a pyrroloquinoline quinone-dependent D-sorbitol dehydrogenase. Gene disruption and complementation experiments demonstrated that this enzyme is responsible for the oxidation of 1-(2-hydroxyethyl) amino-1-deoxy-D-sorbitol (1NSL) to 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose (6NSE), which is the precursor of an antidiabetic drug, miglitol. |
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Authors:
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Xue-Peng Yang; Liu-Jing Wei; Jin-Ping Lin; Bo Yin; Dong-Zhi Wei |
Publication Detail:
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Type: Journal Article Date: 2008-05-23 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 74 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-08-12 Completed Date: 2008-09-19 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 5250-3 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Bioreactor Engineering, East China University of Science and Biotechnology, Mei-Long 130, Shanghai 200237, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Bacterial Proteins / isolation & purification, metabolism* Genetic Complementation Test Genetic Vectors Gluconobacter oxydans / enzymology*, genetics L-Iditol 2-Dehydrogenase / isolation & purification, metabolism* Membrane Proteins / metabolism Oxidation-Reduction PQQ Cofactor / metabolism* Plasmids Sorbitol / metabolism Sorbose / analogs & derivatives*, biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose; 0/Bacterial Proteins; 0/Membrane Proteins; 3615-39-2/Sorbose; 50-70-4/Sorbitol; 72909-34-3/PQQ Cofactor; EC 1.1.1.14/L-Iditol 2-Dehydrogenase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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