| Membrane aging during cell growth ascertained by Laurdan generalized polarization. | |
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MedLine Citation:
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PMID: 1397095 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The sensitivity of the fluorescent probe Laurdan to the phase state of lipids has been utilized to detect modifications in the composition and physical state of cell membranes during cell growth. In phospholipid vesicles, the Laurdan emission spectrum shows a 50-nm red shift by passing from the gel to the liquid-crystalline phase. The Generalized Polarization (GP) value has been used for the data treatment instead of the ratiometric method common in investigations utilizing other fluorescent probes that display spectral sensitivity to medium properties. The GP value can be measured easily and quickly and possesses all the properties of "classical" polarization, including the additivity rule. Once Laurdan limiting GP values have been established for the gel and the liquid-crystalline phase of lipids, the quantitative determination of coexisting phases in natural samples is possible. In the present work the observation of a relevant decrease in the fractional intensity of the liquid-crystalline phase in K562 cell membranes during 5 days of asynchronous growth is reported. A decrease in the "fluidity" of cell membranes in K562 cells kept in culture for several months is also reported. The procedure developed for labeling cell membranes with Laurdan is reported and the influence of cell metabolism on fluorescence parameters is discussed. Also discussed is the influence of cholesterol on Laurdan GP. |
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Authors:
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T Parasassi; M Di Stefano; G Ravagnan; O Sapora; E Gratton |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Experimental cell research Volume: 202 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1992 Oct |
Date Detail:
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Created Date: 1992-10-30 Completed Date: 1992-10-30 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 432-9 Citation Subset: IM |
Affiliation:
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Istituto di Medicina Sperimentale, CNR, Rome, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2-Naphthylamine
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analogs & derivatives* Cell Aging* Cell Division* Cell Line Cell Membrane / physiology* Cholesterol / physiology Fluorescence Polarization Fluorescent Dyes* Kinetics Laurates* |
| Grant Support | |
ID/Acronym/Agency:
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RR03155/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Laurates; 57-88-5/Cholesterol; 74515-25-6/laurdan; 91-59-8/2-Naphthylamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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