Document Detail

Membrane action of tricyclic drugs. Spectroscopic studies of a series of phenothiazines compared with tricyclic antidepressive substances in red cell membrane, using the spin labelling technique.
MedLine Citation:
PMID:  6272818     Owner:  NLM     Status:  MEDLINE    
Previous results were extended by binding studies of drugs on red cell membrane by means of the spin labelling technique. A series of phenothiazines was investigated and compared with several tricyclic antidepressive substances. It was found that phenothiazines at concentrations below 3 mumol/l decisively increase the order parameter of spin label 618, which reports on the interface of the membrane. Thus the fluidity of the interface is decreased. Spin label 616 reports on the hydrophobic membrane interior. Within this hydrophobic phase, the order is decreased by 100 mumol to 1 mmol/l of the phenothiazines. With tricyclic antidepressive substances, a rise of order parameter of spin label 618 is observed, which, however, is less steep. At about 30 mumol/l a maximum is eventually reached. At even higher concentrations of amitriptyline, nortriptyline or imipramine with spin label 616, however, no significant change of order parameter was observed indicating that perturbation of the hydrophobic membrane interior is not sensed. These findings thus corroborate our previous results, revealing that influence of phenothiazines can be measured through the polar and apolar phases of the membrane, while the antidepressive drugs primarily act on the polar phase.
G Zimmer; P Schulze
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Arzneimittel-Forschung     Volume:  31     ISSN:  0004-4172     ISO Abbreviation:  Arzneimittelforschung     Publication Date:  1981  
Date Detail:
Created Date:  1982-01-09     Completed Date:  1982-01-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372660     Medline TA:  Arzneimittelforschung     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  1389-92     Citation Subset:  IM    
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MeSH Terms
Antidepressive Agents, Tricyclic / blood,  pharmacology*
Electron Spin Resonance Spectroscopy
Erythrocyte Membrane / drug effects*,  metabolism
Erythrocytes / drug effects*
Haloperidol / pharmacology
Phenothiazines / blood,  pharmacology*
Spin Labels
Reg. No./Substance:
0/Antidepressive Agents, Tricyclic; 0/Phenothiazines; 0/Spin Labels; 52-86-8/Haloperidol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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