Document Detail


Membrane-bound mucins and mucin terminal glycans expression in idiopathic or Helicobacter pylori, NSAID associated peptic ulcers.
MedLine Citation:
PMID:  22576713     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The ratio of Helicobacter pylori/NSAID-negative gastric ulcers is increasing. Idiopathic gastric ulcers have unique clinical and endoscopic features, and are associated with more bleeding complications and a higher mortality. Alterations in gastric mucin expression and sialylation pattern may be important in ulcer pathogenesis.
AIMS: The purpose of this study was to determine the expression pattern of membrane-bound mucins and side chain sugars in H. pylori associated-, NSAID-, and idiopathic-gastric ulcers.
METHODS: We randomly selected 92 patients with H. pylori (group 1, n = 30), NSAID (group 2, n = 18), combined H. pylori and NSAID associated gastric ulcers (group 3, n = 24), and patients with idiopathic gastric ulcers (group 4, n = 20). Immunohistochemistry for T-cell CD4/CD8, MUC1, MUC4, MUC17, and ECA and SNA lectins staining was performed on sections from the ulcer margins. Inflammation score was assessed according to the Sydney system.
RESULTS: Bleeding and mortality rates were significantly higher in group 4. CD4 positive T cell count was higher in H. pylori positive patients (P = 0.009). Staining intensity of MUC17 was higher in group 1 than in group 4, foveola and glands alike, with 11.50 ± 3.47 versus 6.80 ± 4.02, and 9.61 ± 4.26 versus 7.59 ± 3.26, respectively (P < 0.0001). This was a mirror image with MUC1. SNA lectin staining was increased in group 4, in parallel to MUC1 expression, indicating more abundant α2-6 sialylation in that group.
CONCLUSIONS: Cytoplasmic MUC17 staining was significantly decreased in the cases with idiopathic ulcer. The opposite was demonstrated for MUC1. This observation might be important, since different mucins with altered sialylation patterns likely differ in their protection efficiency against acid and pepsin.
Authors:
Yaron Niv; Doron Boltin; Marisa Halpern; Miriam Cohen; Zohar Levi; Alex Vilkin; Sara Morgenstern; Vahig Manugian; Erica St Lawrence; Pascal Gagneux; Surinder K Batra; Samuel B Ho
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Retracted Publication     Date:  2012-05-11
Journal Detail:
Title:  Digestive diseases and sciences     Volume:  57     ISSN:  1573-2568     ISO Abbreviation:  Dig. Dis. Sci.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-26     Completed Date:  2012-12-28     Revised Date:  2013-08-16    
Medline Journal Info:
Nlm Unique ID:  7902782     Medline TA:  Dig Dis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2535-44     Citation Subset:  AIM; IM    
Affiliation:
Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, 39 Jabotinski Street, 49100 Petach Tikva, Israel. nivyaron80@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Aged, 80 and over
Anti-Inflammatory Agents, Non-Steroidal / adverse effects*
CD4-CD8 Ratio
Female
Gastric Mucins / chemistry,  metabolism*
Gastrointestinal Hemorrhage / etiology
Gene Expression Regulation / physiology
Helicobacter Infections / complications*
Helicobacter pylori / physiology*
Humans
Lectins / genetics,  metabolism
Male
Middle Aged
Peptic Ulcer / etiology*,  metabolism*
Polysaccharides / chemistry,  metabolism
Young Adult
Grant Support
ID/Acronym/Agency:
P30 NS047101/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Gastric Mucins; 0/Lectins; 0/Polysaccharides
Comments/Corrections
Retraction In:
Dig Dis Sci. 2013 Jun;58(6):1812   [PMID:  23712373 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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