Document Detail


Membrane-Bound Alcohol Dehydrogenase Is Essential for Glyceric Acid Production in Acetobacter tropicalis.
MedLine Citation:
PMID:  21852749     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Acetobacter tropicalis NBRC16470 can produce highly enantiomerically pure D-glyceric acid (D-GA; >99 % enantiomeric excess) from glycerol. To investigate whether membrane-bound alcohol dehydrogenase (mADH) is involved in GA production in A. tropicalis, we amplified part of the gene encoding mADH subunit I (adhA) using polymerase chain reaction and constructed an adhA-disrupted mutant of A. tropicalis (ΔadhA). Because ΔadhA did not produce GA, we confirmed that mADH is essential for the conversion of glycerol to GA. We also cloned and sequenced the entire region corresponding to adhA and adhB, which encodes mADH subunit II. The sequences showed high identities (84-86 %) with the equivalent mADH subunits from other Acetobacter spp.
Authors:
Hiroshi Habe; Shun Sato; Tokuma Fukuoka; Dai Kitamoto; Toshiharu Yakushi; Kazunobu Matsushita; Keiji Sakaki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of oleo science     Volume:  60     ISSN:  1347-3352     ISO Abbreviation:  J Oleo Sci     Publication Date:  2011  
Date Detail:
Created Date:  2011-08-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101175339     Medline TA:  J Oleo Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  489-94     Citation Subset:  IM    
Affiliation:
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST).
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