Document Detail


Members of the AP-1 family, c-Jun and c-Fos, functionally interact with JC virus early regulatory protein large T antigen.
MedLine Citation:
PMID:  12692226     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The activating protein 1 (AP-1) family of regulatory proteins is characterized as immediate-early inducible transcription factors which were shown to be activated by a variety of stress-related stimuli and to be involved in numerous biological processes, including cellular and viral gene expression, cell proliferation, differentiation, and tumorigenesis. We have recently demonstrated the involvement of the AP-1 family members c-Jun and c-Fos in transcriptional regulation of the human polyomavirus, JC virus (JCV), genome. Here, we further examined their role in JCV gene regulation and replication through their physical and functional interaction with JCV early regulatory protein large T antigen (T-Ag). Transfection and replication studies indicated that c-Jun and c-Fos can significantly diminish T-Ag-mediated JCV gene transcription and replication. Affinity chromatography and coimmunoprecipitation assays demonstrated that c-Jun and T-Ag physically interact with each other. Results from band shift assays showed that the binding efficiency of c-Jun to the AP-1 site was reduced in the presence of T-Ag. In addition, we have mapped, through the use of a series of deletion mutants, the regions of these proteins which are important for their interaction. While the c-Jun interaction domain of T-Ag is localized to the middle portion of the protein, the T-Ag interacting domain of c-Jun maps to its basic-DNA binding region. Results of transient-transfection assays with various c-Jun mutants and T-Ag expression constructs further confirm the specificity of the functional interaction between c-Jun and T-Ag. Taken together, these data demonstrate that immediate-early inducible transcription factors c-Jun and c-Fos physically and functionally interact with JCV major early regulatory protein large T-Ag and that this interaction modulates JCV transcription and replication in glial cells.
Authors:
Joanne Kim; Stefanie Woolridge; Renato Biffi; Elisa Borghi; Adam Lassak; Pasquale Ferrante; Shohreh Amini; Kamel Khalili; Mahmut Safak
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  77     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-14     Completed Date:  2003-05-27     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5241-52     Citation Subset:  IM    
Affiliation:
Laboratory of Molecular Neurovirology, Center for Neurovirology and Cancer Biology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania 19122, USA.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Polyomavirus Transforming / metabolism*
DNA, Viral / metabolism
Gene Deletion
Gene Expression Regulation, Viral*
Humans
JC Virus / immunology,  metabolism,  physiology*
Neuroglia
Proto-Oncogene Proteins c-fos / genetics,  metabolism*
Proto-Oncogene Proteins c-jun / genetics,  metabolism*
Transcription Factor AP-1 / classification,  metabolism
Transcription, Genetic
Tumor Cells, Cultured
Virus Replication
Chemical
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 0/DNA, Viral; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/Transcription Factor AP-1
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