| Melittin-mediated release of [3H]-oleic acid from E. coli cells is dependent upon heat- and trypsin-sensitive factor(s) in human serum. | |
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MedLine Citation:
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PMID: 10708799 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Synthetic melittin mediated the release of [3H]-oleic acid ([3H]-OA) or its acylated lipids from [3H]-OA-labeled E. coli cells exposed to human serum. This phenomenon was not observed in the absence of serum and was calcium independent. The addition of serum was not required for melittin-mediated lysis of erythrocytes, although lysis was greater in the presence of serum than in its absence (P<0.001). Trypsin treatment of human serum reduced the melittin-mediated release of [3H]-OA/acylated lipids, and this effect was more pronounced upon boiling the serum (P<0.01). A kinetic study showed that maximum release of [3H]-OA/acylated lipids occurred within 3-6 min. Thin layer chromatography (TLC) analysis showed the lipids to be phosphatidyl ethanolamine (PE), phosphatidylethanol (PEt) and phosphatidic acid (PA). There was no detectable level of oleic acid (OA), diacylglycerol (DAG), phosphatidyl choline (PC) or phosphatidyl serine (PS). These findings suggested that a trypsin and heat-sensitive enzyme/factor present in the serum had a role in melittin-mediated action. These findings further showed that melittin activated phospholipase D (PLD), without affecting phospholipase A(2) (PLA(2)) or phospholipase C (PLC) activity. |
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Authors:
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S S Saini; A K Chopra; J W Peterson |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Toxicon : official journal of the International Society on Toxinology Volume: 38 ISSN: 0041-0101 ISO Abbreviation: Toxicon Publication Date: 2000 Aug |
Date Detail:
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Created Date: 2000-05-31 Completed Date: 2000-05-31 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 1307333 Medline TA: Toxicon Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1077-86 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-1070, USA. sssaini@utmb.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Thin Layer Erythrocytes / drug effects, enzymology Escherichia coli / drug effects, metabolism* Hemolysis / drug effects Hot Temperature Humans Indicators and Reagents Lipids / chemistry Melitten / pharmacology* Oleic Acid / metabolism* Trypsin / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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AI21463/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; 0/Lipids; 112-80-1/Oleic Acid; 20449-79-0/Melitten; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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