Document Detail

Melissa officinalis Essential Oil Reduces Plasma Triglycerides in Human Apolipoprotein E2 Transgenic Mice by Inhibiting Sterol Regulatory Element-Binding Protein-1c-Dependent Fatty Acid Synthesis.
MedLine Citation:
PMID:  22279139     Owner:  NLM     Status:  Publisher    
We investigated the hypolipidemic effects of Melissa officinalis essential oil (MOEO) in human APOE2 transgenic mice and lipid-loaded HepG2 cells. Plasma TG concentrations were significantly less in APOE2 mice orally administered MOEO (12.5 μg/d) for 2 wk than in the vehicle-treated group. Cellular TG and cholesterol concentrations were also significantly decreased in a dose- (400 and 800 mg/L) and time- (12 and 24 h) dependent manner in HepG2 cells stimulated with MOEO compared with controls. Mouse hepatic transcriptome analysis suggested MOEO feeding altered several lipid metabolic pathways, including bile acid and cholesterol synthesis and fatty acid metabolism. In HepG2 cells, the rate of fatty acid oxidation, as assessed using [1-(14)C]palmitate, was unaltered; however, the rate of fatty acid synthesis quantified with [1-(14)C]acetate was significantly reduced by treatment of 400 and 800 mg/L MOEO compared with untreated controls. This reduction was due to the decreased expression of SREBP-1c and its responsive genes in fatty acid synthesis, including FAS, SCD1, and ACC1. Subsequent chromatin immunoprecipitation analysis further demonstrated that the binding of p300/CBP-associated factor, a coactivator of SREBP-1c, and histone H3 lysine 14 acetylation at the FAS, SCD1, and ACC1 promoters were significantly reduced in the livers of APOE2 mice and HepG2 cells with MOEO compared with their controls. Additionally, MOEO stimulation in HepG2 cells induced bile acid synthesis and reduced the nuclear form of SREBP-2, a key transcription factor in hepatic cholesterol synthesis. These findings suggest that the intake of phytochemicals with pleasant scent could have beneficial metabolic effects.
Hee-Jin Jun; Ji Hae Lee; Yaoyao Jia; Minh-Hien Hoang; Hanna Byun; Kyoung Heon Kim; Sung-Joon Lee
Related Documents :
1213669 - Studies on the chemistry of arterial wall, xvii. metabolic characteristics of different...
21110089 - Functions of chondroitin sulfate and heparan sulfate in the developing brain.
22956279 - Animal bone char solubilization with itaconic acid produced by free and immobilized asp...
8674529 - Interferon-gamma inhibits 35s incorporation in heparan sulfate synthesized by human ski...
12201999 - Bone morphogenetic proteins-2, -12, and -13 modulate in vitro development of engineered...
4071539 - Glycosaminoglycans in brush border membranes from rabbit small intestine.
2111169 - Determinants of visual pigment absorbance: role of charged amino acids in the putative ...
12898349 - Decreased lysophosphatidylcholine/phosphatidylcholine ratio in cerebrospinal fluid in a...
17217469 - The arabidopsis thaliana sulfiredoxin is a plastidic cysteine-sulfinic acid reductase i...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-25
Journal Detail:
Title:  The Journal of nutrition     Volume:  -     ISSN:  1541-6100     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Stearidonic Acid Raises Red Blood Cell Membrane Eicosapentaenoic Acid.
Next Document:  Mechanisms of Action of (n-3) Fatty Acids.