Document Detail


Melatonin restores the basal level of lipid peroxidation in rat tissues exposed to potassium bromate in vitro.
MedLine Citation:
PMID:  20588237     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Potassium bromate (KBrO3) is a prooxidant and carcinogen. Melatonin is a highly effective antioxidant. Indole-3-propionic acid (IPA; indole substance) and propylothiouracil (PTU; antithyroid drug) reveal some antioxidative effects. The aim of the study was to evaluate KBrO3-induced lipid peroxidation (LPO) in vitro in tissues collected from control or melatonin-treated rats, and to compare potential preventive effects of melatonin, IPA and PTU. MATERIALS AND METHODS: Kidney, liver and lung homogenates from either control or melatonin-pretreated rats (0.0645 mmol/kg b.w., i.p., twice daily, 10 days) were incubated in the presence of KBrO3 (0.1, 0.5, 1.0, 2.5, 5.0, 10.0 mM). Then, control lung homogenates were incubated with KBrO3 (10.0 mM) together with melatonin (0.01, 0.1, 0.5, 1.0, 5.0, 7.5 mM), or with IPA or with PTU. LPO products (malondialdehyde+4-hydroxyalkenals) were measured spectrophotometrically. RESULTS: Melatonin injections prevented KBrO3-induced LPO in lung homogenates. Melatonin, IPA and PTU, used in vitro, reduced KBrO3-induced LPO in control lungs. Unexpectedly, KBrO3 caused a concentration-dependent decrease in LPO in liver and kidney homogenates from control but not from melatonin-treated rats. CONCLUSIONS: Potassium bromate-induced LPO in the rat lung homogenates suggests that the lung may be the target for this carcinogen. An exposure of organisms to melatonin decreases tissue sensitivity to KBrO3-induced damage, possibly by restoring the oxidative balance.
Authors:
Magdalena Stasiak; Krzysztof Zasada; Andrzej Lewinski; Małgorzata Karbownik-Lewinska
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuro endocrinology letters     Volume:  31     ISSN:  0172-780X     ISO Abbreviation:  Neuro Endocrinol. Lett.     Publication Date:  2010  
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-10-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008373     Medline TA:  Neuro Endocrinol Lett     Country:  Sweden    
Other Details:
Languages:  eng     Pagination:  363-9     Citation Subset:  IM    
Affiliation:
Department of Endocrinology and Metabolic Diseases, Polish Mother's Memorial Hospital - Research Institute of Lodz, Lodz, Poland. MKarbownik@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / pharmacology*
Bromates / adverse effects,  antagonists & inhibitors*
Dose-Response Relationship, Drug
Indoles / pharmacology
Kidney / drug effects,  metabolism
Lipid Peroxidation / drug effects*
Liver / drug effects,  metabolism
Lung / drug effects,  metabolism
Melatonin / pharmacology*
Propylthiouracil / pharmacology
Rats
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Bromates; 0/Indoles; 51-52-5/Propylthiouracil; 73-31-4/Melatonin; 7758-01-2/potassium bromate; 830-96-6/indolepropionic acid

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