Document Detail

Melatonin protects against hydrogen peroxide-induced cell death signaling in SH-SY5Y cultured cells: involvement of nuclear factor kappa B, Bax and Bcl-2.
MedLine Citation:
PMID:  16879316     Owner:  NLM     Status:  MEDLINE    
Oxidative stress is defined as a disturbance in the prooxidant-antioxidant balance, leading to potential cell damage. Reactive oxygen species such as superoxide radicals, hydroxyl radicals and hydrogen peroxide may act also as secondary intermediaries in intracellular signaling leading to cell death. The neuroprotective effect of melatonin has been observed both in vivo and in vitro. The objective of this research, therefore, was to better understand the cellular mechanisms of neuronal cell degeneration induced via oxidative stress and the protective roles of melatonin on this cell death. In the present study, the effects of melatonin on H(2)O(2)-induced neuronal cell degeneration in human dopaminergic neuroblastoma SH-SY5Y cultured cells were investigated. The results showed that H(2)O(2) significantly decreased cell viability and melatonin reversed the toxic effects of H(2)O(2). An inhibition of caspase enzyme activity by Ac-DEVD-CHO, a caspase-3 inhibitor, significantly increased cell viability in H(2)O(2)-treated cells. The phosphorylation of transcription factors, nuclear factor kappa B (NF-kappaB) was increased in H(2)O(2)-treated cells and this effect was abolished by melatonin. Translocation of phosphorylated NF-kappaB to perinuclear and nuclear sites, estimated using immunofluorescence, occurred to a greater extent in H(2)O(2)-treated cells than in untreated control cells and again this effect was abolished by melatonin. In addition, induction of Bcl-2 and Bax proteins was demonstrated in SH-SY5Y cultured cells treated with H(2)O(2), whereas the induction of Bax but not Bcl-2 was diminished by melatonin. In light of these finding, it is possible that the antioxidative stress effect of melatonin associated with inhibition of Bax expression, may offer a means of treating neuronal degeneration and disease.
Banthit Chetsawang; Chorkaew Putthaprasart; Pansiri Phansuwan-Pujito; Piyarat Govitrapong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pineal research     Volume:  41     ISSN:  0742-3098     ISO Abbreviation:  J. Pineal Res.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-01     Completed Date:  2006-09-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8504412     Medline TA:  J Pineal Res     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  116-23     Citation Subset:  IM    
Neuro-Behavioural Biology Center, Institute of Science and Technology for Research and Development, Mahidol University, Salaya, Nakornpathom, Thailand.
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MeSH Terms
Active Transport, Cell Nucleus
Antioxidants / pharmacology*
Blotting, Western
Caspase 3
Caspases / antagonists & inhibitors,  metabolism
Cell Death / drug effects*
Cell Line, Tumor
Hydrogen Peroxide / pharmacology*
Melatonin / pharmacology*
NF-kappa B / metabolism
Neurons / cytology*
Neuroprotective Agents / pharmacology*
Oxidative Stress
Proto-Oncogene Proteins c-bcl-2 / metabolism
Signal Transduction
bcl-2-Associated X Protein / metabolism
Reg. No./Substance:
0/Antioxidants; 0/NF-kappa B; 0/Neuroprotective Agents; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 73-31-4/Melatonin; 7722-84-1/Hydrogen Peroxide; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

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