Document Detail

Melatonin in mice: rhythms, response to light, adrenergic stimulation, and metabolism.
MedLine Citation:
PMID:  11792644     Owner:  NLM     Status:  MEDLINE    
There has been relatively little research conducted on pineal melatonin production in laboratory mice, in part, due to the lack of appropriate assays. We studied the pineal and plasma rhythm, response to light, adrenergic stimulation, and metabolism of melatonin in CBA mice. With the use of a sensitive and specific melatonin RIA, melatonin was detected in the pineal glands at all times of the day >21 fmol/gland in CBA mice but not in C57Bl mice. Both plasma and pineal melatonin levels peaked 2 h before dawn in a 12:12-h light-dark photoperiod (162 +/- 31 pM and 1,804 +/- 514 fmol/gland, respectively). A brief light pulse (200 lx/15 min), 2 h before lights on, suppressed both plasma and pineal melatonin to near basal levels within 30 min. Exposure to light pulses 4 h after lights off or 2 h before lights on resulted in delays and advances, respectively, in the early morning decline of plasma and pineal melatonin on the next cycle. Administration of the beta-adrenergic agonist isoproterenol (20 mg/kg) 2 and 4 h after lights on in the morning resulted in a fivefold increase in plasma and pineal melatonin 2.5 to 3 h after the first injection. In the mouse, unlike the rat, melatonin was shown to be metabolized almost exclusively to 6-glucuronylmelatonin rather than 6-sulphatoxymelatonin. These studies have shown that the appropriate methodological tools are now available for studying melatonin rhythms in mice.
D J Kennaway; A Voultsios; T J Varcoe; R W Moyer
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  282     ISSN:  0363-6119     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-01-16     Completed Date:  2002-02-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R358-65     Citation Subset:  IM    
Department of Obstetrics and Gynaecology, Adelaide University, Medical School, Adelaide, South Australia 5005.
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MeSH Terms
Adrenergic beta-Agonists / pharmacology*
Circadian Rhythm / physiology*
Isoproterenol / pharmacology*
Melatonin / analogs & derivatives*,  blood*,  pharmacology,  urine
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred CBA
Pineal Gland / drug effects,  metabolism*
Species Specificity
Reg. No./Substance:
0/Adrenergic beta-Agonists; 2208-40-4/6-sulfatoxymelatonin; 73-31-4/Melatonin; 7683-59-2/Isoproterenol

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