| Melatonin attenuates 1-methyl-4-phenylpyridinium-induced PC12 cell death. | |
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MedLine Citation:
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PMID: 15659124 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To explore the effect of melatonin on PC12 cell death induced by 1-methyl-4-phenylpyridinium (MPP+). METHODS: MTT assay, lactate dehydrogenase (LDH) efflux assay, and immunohistochemistry methods were used to measure neurotoxicity of PC12 cells treated acutely with MPP+ in low glucose and high glucose conditions, and to assess the neuroprotective effect of melatonin on PC12 cell death induced by MPP+. RESULTS: In a low glucose condition, MPP+ significantly induced PC12 cell death, which showed time and concentration dependence. In a serum-free low glucose condition, the percentages of viability of cells treated with MPP+ for 12, 24, 48, 72, and 96 h were 85.1%, 75.4%, 64.9%, 28.15%, and 9%, respectively. The level of LDH in the culture medium increased and tyrosine hydroxylase positive (TH+) cell count decreased. However, in a serum-free high glucose condition, MPP+ did not significantly induce PC12 cell death compared with control at various concentrations and time regimens. When the cells were preincubated with melatonin 250 micromol/L for 48, 72, and 96 h in a serum-free low glucose condition, cell survival rate significantly increased to 78.1%, 58.8%, and 31.6%, respectively. Melatonin abolished the LDH leakage of cells treated with MPP+ and increased TH+ cells count. CONCLUSION: MPP+ caused concentration-dependent PC12 cell death. The level of glucose was an important factor to MPP+ induced dopaminergic PC12 cell death. Low glucose level could potentiate MPP+ toxicity, while high glucose level could reduce the toxicity. In addition, melatonin attenuated PC12 cell death induced by MPP+. |
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Authors:
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Jin-feng Bao; Ren-gang Wu; Xiao-ping Zhang; Yan Song; Chang-ling Li |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta pharmacologica Sinica Volume: 26 ISSN: 1671-4083 ISO Abbreviation: Acta Pharmacol. Sin. Publication Date: 2005 Jan |
Date Detail:
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Created Date: 2005-01-20 Completed Date: 2005-06-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100956087 Medline TA: Acta Pharmacol Sin Country: England |
Other Details:
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Languages: eng Pagination: 117-23 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, School of Pharmaceutical Sciences, Beijing 100083, China. srgltlsn93@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Methyl-4-phenylpyridinium
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antagonists & inhibitors*,
toxicity Animals Cell Death / drug effects Cell Survival / drug effects Culture Media, Serum-Free Glucose / administration & dosage, pharmacology* L-Lactate Dehydrogenase / metabolism Melatonin / pharmacology* Neuroprotective Agents / pharmacology* PC12 Cells / drug effects, metabolism Rats Tyrosine 3-Monooxygenase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Serum-Free; 0/Neuroprotective Agents; 48134-75-4/1-Methyl-4-phenylpyridinium; 50-99-7/Glucose; 73-31-4/Melatonin; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.14.16.2/Tyrosine 3-Monooxygenase |
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