| Melatonin reprogrammes proteomic profile in light-exposed retina in vivo. | |
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MedLine Citation:
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PMID: 20434483 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Melatonin, a small organic molecule synthesized by the pineal gland and the retina, has a variety of physiologic functions such as circadian clock pacemaker and antioxidant. Retinal melatonin is down-regulated by light and is barely detectable during the day. The absence of melatonin in the retina during prolonged light exposure may contribute to light-induced retinal degeneration. We sought to investigate the impact of melatonin in the light-exposed retina using proteomic approaches. We exposed mice to either light (250-300lux) for 12h followed by 12h of darkness or the same intensity of continuous light for 7 days. In half of the animals exposed to continuous light, melatonin was injected each night. Proteomic analysis of the retina from these three groups of animals showed that five proteins prominently up-regulated by constant light were down-regulated by melatonin treatment. These five proteins were identified as vimentin, serine/threonine-protein phosphatase 2A, Rab GDP dissociation inhibitor alpha, guanine nucleotide-binding protein G(o) alpha, and retinaldehyde-binding protein. These five proteins are known to be involved in several cellular processes that may contribute to light-induced retinal degeneration. Identification of melatonin target proteins in our study provides a basis for future studies on melatonin's potential in preventing or treating light-induced retinal degeneration. |
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Authors:
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Ruonan Zhang; William J M Hrushesky; Patricia A Wood; Sung Haeng Lee; Richard C Hunt; Wan Jin Jahng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-29 |
Journal Detail:
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Title: International journal of biological macromolecules Volume: 47 ISSN: 1879-0003 ISO Abbreviation: Int. J. Biol. Macromol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-08 Completed Date: 2010-10-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7909578 Medline TA: Int J Biol Macromol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 255-60 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Ophthalmology, University of South Carolina, Columbia, SC 29208, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Darkness Dose-Response Relationship, Radiation Female Gene Expression Regulation / drug effects, radiation effects Light* Melatonin / pharmacology* Mice Protein Processing, Post-Translational / drug effects, radiation effects Proteome / metabolism* Proteomics Retina / drug effects*, metabolism, radiation effects* Time Factors Vimentin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Proteome; 0/Vimentin; 73-31-4/Melatonin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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