Document Detail


Melanosome-iron interactions within retinal pigment epithelium-derived cells.
MedLine Citation:
PMID:  22883111     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Melanosomes were recently shown to protect ARPE-19 cells, a human retinal pigment epithelium (RPE) cell line, against oxidative stress induced by hydrogen peroxide. One postulated mechanism of antioxidant action of melanin is its ability to bind metal ions. The aim here was to determine whether melanosomes are competent to bind iron within living cells, exhibiting a property previously shown only in model systems. The outcomes indicate retention of prebound iron and accumulation of iron by granules after iron delivery to cells via the culture medium, as determined by both colorimetric and electron spin resonance analyses for bound-to-melanosome iron. Manipulation of iron content did not affect the pigment's ability to protect cells against H(2) O(2) , but the function of pigment granules within RPE cells should be extended beyond a role in light irradiation to include participation in iron homeostasis.
Authors:
Patrycja Kaczara; Mariusz Zaręba; Anja Herrnreiter; Christine M B Skumatz; Andrzej Ządło; Tadeusz Sarna; Janice M Burke
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-01
Journal Detail:
Title:  Pigment cell & melanoma research     Volume:  25     ISSN:  1755-148X     ISO Abbreviation:  Pigment Cell Melanoma Res     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-22     Completed Date:  2013-09-03     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  101318927     Medline TA:  Pigment Cell Melanoma Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  804-14     Citation Subset:  IM    
Copyright Information:
© 2012 John Wiley & Sons A/S.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoferritins / metabolism
Cell Line
Densitometry
Electron Spin Resonance Spectroscopy
Extracellular Space / drug effects,  metabolism
Humans
Hydrogen Peroxide / pharmacology
Iron / metabolism*,  pharmacology
Melanosomes / drug effects,  metabolism*
Microscopy, Phase-Contrast
Oxidative Stress / drug effects
Phagocytosis / drug effects
Retinal Pigment Epithelium / cytology*,  drug effects,  metabolism*
Sus scrofa
Grant Support
ID/Acronym/Agency:
C06 RR-RR016511/RR/NCRR NIH HHS; P30EY01931/EY/NEI NIH HHS; R01 EY019664/EY/NEI NIH HHS; R01EY019664/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
9013-31-4/Apoferritins; BBX060AN9V/Hydrogen Peroxide; E1UOL152H7/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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