Document Detail

Melanoma cell lines from different stages of progression and their biological and molecular analyses.
MedLine Citation:
PMID:  9578415     Owner:  NLM     Status:  MEDLINE    
The biological and molecular characteristics of cell lines from metastatic melanomas have been extensively studied but less is known about cells from the biologically earliest stage of primary melanoma. The overall success rate of establishing permanent cell lines from such lesions is only 10% of that for biologically late primary or metastatic melanomas, although our laboratory now has eight cell lines available. The cells are immortal but show reduced or no proliferation in soft agar and immunodeficient mice when compared with primary melanomas from the biologically advanced vertical growth phase. Metastatic melanoma cell lines from patients with familial melanoma or xeroderma pigmentosum are biologically similar to those from patients with spontaneous melanomas. Irrespective of the malignant stages, deletions and mutations can occur in exons 1-3 of the p16INK4A gene. DNA fingerprinting was then employed to demonstrate the uniqueness of individual cell lines and to confirm the identity of cell lines derived from same patients. These cell lines are an excellent resource to investigate melanoma progression.
K Satyamoorthy; E DeJesus; A J Linnenbach; B Kraj; D L Kornreich; S Rendle; D E Elder; M Herlyn
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Melanoma research     Volume:  7 Suppl 2     ISSN:  0960-8931     ISO Abbreviation:  Melanoma Res.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1998-06-09     Completed Date:  1998-06-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9109623     Medline TA:  Melanoma Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  S35-42     Citation Subset:  IM    
The Wistar Institute, Philadelphia, PA 19104, USA.
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MeSH Terms
Cell Division / physiology
Chromosomes, Human, Pair 9
Cyclin-Dependent Kinase Inhibitor p16 / genetics
DNA Fingerprinting
DNA, Neoplasm / analysis*
Disease Progression
Gene Deletion
Melanoma / genetics*,  pathology*,  secondary
Neoplasm Staging
Polymerase Chain Reaction
Tumor Cells, Cultured*
Grant Support
Reg. No./Substance:
0/Cyclin-Dependent Kinase Inhibitor p16; 0/DNA, Neoplasm

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