Document Detail


Melanogenesis and evidence for melanosome transport to the plasma membrane in a CD83 teleost leukocyte cell line.
MedLine Citation:
PMID:  16704455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Visceral organs of ectothermic vertebrates harbour melanin-containing leukocytes termed melanomacrophages. These cells are thought to participate in immune reactions and free-radical trapping. In teleosts, the melanin-producing ability of melanomacrophages has hitherto not been confirmed by molecular techniques. Here, a leukocyte marker and the apparatus for melanosome production and transport were investigated in an Atlantic salmon (Salmo salar) pronephros-derived mononuclear leukocyte (SHK-1) cell line. The SHK-1 cells expressed transcripts specific for a mammalian CD83 homologue, a standard surface marker for activated or differentiated dendritic cells, and dopachrome tautomerase/tyrosinase-related protein-2, a melanocyte specific enzyme essential for melanin production. Reduction potential of melanin or its precursors was demonstrated histochemically after prolonged cultivation. Ultrastructural investigations revealed tyrosinase and acid phosphate activity in identical organelles and BSA-gold co-localized with multilamellar melanosomes after 2 h internalization. Apparently, melanosomes were transported and released through periodically occurring tubules fusing with the plasma membrane. Video monitoring revealed filopodia and macropinocytosis. These results showed that the SHK-1 cell line is capable of melanogenesis and melanosome secretion. Melanin-producing cells in teleost pronephros may represent a distinct CD83(+) leukocyte population consisting of phylogenetically relict multifunctional cells. This is the first report of a melanin-producing leukocyte cell-line.
Authors:
Erlend Haugarvoll; Jim Thorsen; Morten Laane; Qirong Huang; Erling Olaf Koppang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society     Volume:  19     ISSN:  0893-5785     ISO Abbreviation:  Pigment Cell Res.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-17     Completed Date:  2006-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8800247     Medline TA:  Pigment Cell Res     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  214-25     Citation Subset:  IM    
Affiliation:
Institute of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, Oslo. erlend.haugarvoll@veths.no
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ339139;  DQ339140;  DQ339141
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD / genetics,  metabolism*
Base Sequence
Biological Transport
Cell Line
Cell Membrane / physiology*
Immunoglobulins / genetics,  metabolism*
Intramolecular Oxidoreductases / metabolism
Leukocytes / cytology*,  physiology
Melanins / biosynthesis*,  physiology
Melanosomes / physiology*,  ultrastructure
Membrane Glycoproteins / genetics,  metabolism*
Microtubules
Molecular Sequence Data
Monophenol Monooxygenase / metabolism
Salmon / physiology
Transport Vesicles / physiology,  ultrastructure
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/CD83 antigen; 0/Immunoglobulins; 0/Melanins; 0/Membrane Glycoproteins; EC 1.14.18.1/Monophenol Monooxygenase; EC 5.3.-/Intramolecular Oxidoreductases; EC 5.3.3.12/dopachrome isomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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