Document Detail

Melanocortin receptors form constitutive homo- and heterodimers.
MedLine Citation:
PMID:  15582585     Owner:  NLM     Status:  MEDLINE    
A significant number of G protein-coupled receptors are shown to form homo- or heterodimers/oligomers, and oligomerization of GPCRs may be a quite general phenomenon. We have here explored the possibility that the two closely related human melanocortin receptor 1 (MC(1)R) and melanocortin receptor 3 (MC(3)R) form dimers. Using bioluminescence resonance energy transfer (BRET(2)) we demonstrate that MC(1) and MC(3)Rs form homo- and heterodimers, when expressed in Cos-7 cells. Treatment with agonist, partial agonist or antagonists did not modify the BRET(2) signal for any of the receptor pairs studied, suggesting that the dimerization is not regulated by ligand binding. Rather our results indicate that melanocortin receptors exist as constitutively pre-formed dimers.
Ilona Mandrika; Ramona Petrovska; Jarl Wikberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  326     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-12-07     Completed Date:  2005-01-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  349-54     Citation Subset:  IM    
Division of Pharmacology, Department of Pharmaceutical Biosciences, Uppsala University, SE-751 24 Uppsala, Sweden.
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MeSH Terms
COS Cells
Cercopithecus aethiops
Energy Transfer
Protein Structure, Quaternary
Radioligand Assay
Receptor, Melanocortin, Type 1 / chemistry*,  genetics,  metabolism*
Receptor, Melanocortin, Type 3 / chemistry*,  genetics,  metabolism*
Spectrometry, Fluorescence
Reg. No./Substance:
0/Ligands; 0/Receptor, Melanocortin, Type 1; 0/Receptor, Melanocortin, Type 3

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