Document Detail

Melanocortin receptors form constitutive homo- and heterodimers.
MedLine Citation:
PMID:  15582585     Owner:  NLM     Status:  MEDLINE    
A significant number of G protein-coupled receptors are shown to form homo- or heterodimers/oligomers, and oligomerization of GPCRs may be a quite general phenomenon. We have here explored the possibility that the two closely related human melanocortin receptor 1 (MC(1)R) and melanocortin receptor 3 (MC(3)R) form dimers. Using bioluminescence resonance energy transfer (BRET(2)) we demonstrate that MC(1) and MC(3)Rs form homo- and heterodimers, when expressed in Cos-7 cells. Treatment with agonist, partial agonist or antagonists did not modify the BRET(2) signal for any of the receptor pairs studied, suggesting that the dimerization is not regulated by ligand binding. Rather our results indicate that melanocortin receptors exist as constitutively pre-formed dimers.
Ilona Mandrika; Ramona Petrovska; Jarl Wikberg
Related Documents :
15956755 - Evolution of the signaling system in relaxin-family peptides.
18562325 - Lysophospholipid signaling in the function and pathology of the reproductive system.
15158755 - Sphingosine-1-phosphate receptors: receptor specificity versus functional redundancy.
22895715 - Plasticity of metabotropic glutamate receptor-dependent long-term depression in the ant...
12417645 - Barbiturates induce mitochondrial depolarization and potentiate excitotoxic neuronal de...
20005925 - Potential use of melatonergic drugs in analgesia: mechanisms of action.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  326     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-12-07     Completed Date:  2005-01-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  349-54     Citation Subset:  IM    
Division of Pharmacology, Department of Pharmaceutical Biosciences, Uppsala University, SE-751 24 Uppsala, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
COS Cells
Cercopithecus aethiops
Energy Transfer
Protein Structure, Quaternary
Radioligand Assay
Receptor, Melanocortin, Type 1 / chemistry*,  genetics,  metabolism*
Receptor, Melanocortin, Type 3 / chemistry*,  genetics,  metabolism*
Spectrometry, Fluorescence
Reg. No./Substance:
0/Ligands; 0/Receptor, Melanocortin, Type 1; 0/Receptor, Melanocortin, Type 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Age-dependent suppression of SERCA2a mRNA in pediatric atrial myocardium.
Next Document:  Functional preservation of duplicated pair for RSVS III gene in the REST locus of rat 3q42.