Document Detail


Melagenine modulates proliferation and differentiation of melanoblasts.
MedLine Citation:
PMID:  18636173     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Melagenine, extracted from human placenta, has been shown to be effective in treating patients with vitiligo, yet the mechanisms of melagenine in inducing the repigmentation of vitiligo patients have not been fully investigated. Recent studies have suggested that melagenine stimulates melanocyte proliferation and melanogenesis. In this study, we utilized the NCCmelb4M5 melanoblast cell line to investigate the effects of melagenine on proliferation and differentiation of immature melanocytes or melanoblasts. NCCmelb4M5 cells were treated with different concentrations of melagenine (50-400 microg/ml), and MTT assay was performed to evaluate the effects of melagenine on proliferation of melanoblasts. RT-PCR and Western blotting were used to determine the expression of c-KIT and tyrosinase (TYR). Our results show that melagenine stimulates proliferation of NCCmelb4M5 cells in a dose-dependent manner with an optimal concentration of 100 microg/ml. Multipolar and highly branched dendritic network, as well as cluster-like growing cell assembly were visible in melagenine-treated NCCmelb4M5 cells. Melagenine induced expression of c-KIT, TYR and MITF. Our results provide insights into the molecular mechanism of the beneficial effect of melagenine in the treatment of vitiligo.
Authors:
Dekuang Zhao; Yongwei Li; Ping Wang; Miaoni Zhou; Cuiping Guan; Xiaodong Wei; Yinsheng Wan; Aie Xu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of molecular medicine     Volume:  22     ISSN:  1107-3756     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-18     Completed Date:  2008-09-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  193-7     Citation Subset:  IM    
Affiliation:
Department of Dermatology, the Third People's Hospital of Hangzhou, Hangzhou, PR China.
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MeSH Terms
Descriptor/Qualifier:
Cell Differentiation / drug effects*
Cell Line
Cell Proliferation / drug effects*
Cell Shape
Humans
Lipoproteins / pharmacology*,  therapeutic use
Melanocytes / cytology,  drug effects*,  physiology*
Microphthalmia-Associated Transcription Factor / genetics,  metabolism
Placental Extracts / pharmacology*,  therapeutic use
Vitiligo / drug therapy
Grant Support
ID/Acronym/Agency:
P20 RR016457/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Lipoproteins; 0/Melagenine; 0/Microphthalmia-Associated Transcription Factor; 0/Placental Extracts

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