| Mek1 suppression of meiotic double-strand break repair is specific to sister chromatids, chromosome autonomous and independent of Rec8 cohesin complexes. | |
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MedLine Citation:
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PMID: 20421598 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During meiosis, recombination is directed to occur between homologous chromosomes to create connections necessary for proper segregation at meiosis I. Partner choice is determined at the time of strand invasion and is mediated by two recombinases: Rad51 and the meiosis-specific Dmc1. In budding yeast, interhomolog bias is created in part by the activity of a meiosis-specific kinase, Mek1, which is localized to the protein cores of condensed sister chromatids. Analysis of meiotic double-strand break (DSB) repair in haploid and disomic haploid strains reveals that Mek1 suppresses meiotic intersister DSB repair by working directly on sister chromatids. Rec8 cohesin complexes are not required, however, either for suppression of intersister DSB repair or for the repair itself. Regulation of DSB repair in meiosis is chromosome autonomous such that unrepaired breaks on haploid chromosomes do not prevent interhomolog repair between disomic homologs. The pattern of DSB repair in haploids containing Dmc1 and/or Rad51 indicates that Mek1 acts on Rad51-specific recombination processes. |
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Authors:
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Tracy L Callender; Nancy M Hollingsworth |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-04-26 |
Journal Detail:
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Title: Genetics Volume: 185 ISSN: 1943-2631 ISO Abbreviation: Genetics Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-27 Completed Date: 2010-11-16 Revised Date: 2011-07-19 |
Medline Journal Info:
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Nlm Unique ID: 0374636 Medline TA: Genetics Country: United States |
Other Details:
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Languages: eng Pagination: 771-82 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794-5215, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle Proteins
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genetics,
metabolism Chromatids / genetics* Chromosomal Proteins, Non-Histone / genetics*, metabolism DNA Breaks, Double-Stranded* DNA Repair* MAP Kinase Kinase 1 / genetics, metabolism* Meiosis / genetics* Recombination, Genetic Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae Proteins / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM50717/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Chromosomal Proteins, Non-Histone; 0/REC8 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/cohesins; EC 2.7.12.2/MAP Kinase Kinase 1 |
| Comments/Corrections | |
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