Document Detail

Meiotic spindle recovery is faster in vitrification of human oocytes compared to slow freezing.
MedLine Citation:
PMID:  18675965     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To investigate spindle behavior during and after slow freezing at room temperature (RT) and vitrification at different temperatures. DESIGN: Randomized, comparative study. SETTING: University hospital. PATIENT(S): Patients undergoing IVF treatment volunteered for the study and donated part of their supernumerary oocytes. INTERVENTION(S): Metaphase II oocytes were divided into group A: slow freezing RT /thawing RT; group B: vitrification RT/warming RT; group C: vitrification RT/warming 37 degrees C; and group D: vitrification 37 degrees C/warming 37 degrees C. Spindle presence was evaluated at each step of the four procedures and in culture. MAIN OUTCOME MEASURE(S): Cumulative spindle recovery rate comparing warming phase of the three vitrification groups and culture phase among the four groups. RESULT(S): During warming, the three vitrification groups showed a significantly fast spindle recovery rate compared to the thawing of the slow freezing group. A progressively significant fast cumulative recovery rate was observed in the three vitrification groups by increasing the number of phases at physiological temperature (hazard rate = 2.68; 95% confidence interval 1.71-4.02). CONCLUSION(S): The present study demonstrates that spindle recovery is faster in vitrification than in slow freezing. These data support a possible protective effect of vitrification/warming at 37 degrees C on the meiotic spindle structure and, therefore, on the subsequent clinical outcome of the procedure, although comparative clinical studies are needed.
Patrizia Maria Ciotti; Eleonora Porcu; Leonardo Notarangelo; Otello Magrini; Antonia Bazzocchi; Stefano Venturoli
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Publication Detail:
Type:  Journal Article     Date:  2008-08-03
Journal Detail:
Title:  Fertility and sterility     Volume:  91     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-01     Completed Date:  2009-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2399-407     Citation Subset:  IM    
Reproductive Medicine Unit, IVF and Infertility Centre, S.Orsola-Malpighi University Hospital, University of Bologna, Bologna, Italy.
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MeSH Terms
Cell Culture Techniques / methods
Cytoskeleton / physiology,  ultrastructure
Fertilization in Vitro / methods
Meiosis / physiology*
Oocyte Retrieval / methods
Oocytes / cytology*,  physiology*
Random Allocation
Tissue Preservation / methods

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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