Document Detail


Meiotic behaviours of chromosomes and microtubules in budding yeast: relocalization of centromeres and telomeres during meiotic prophase.
MedLine Citation:
PMID:  9813109     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Meiosis is a process of universal importance in eukaryotic organisms, generating variation in the heritable haploid genome by recombination and re-assortment of chromosomes. The intranuclear movement of chromosomes is expected to achieve pairing and recombination of homologous chromosomes during meiosis. Meiosis in the budding yeast Saccharomyces cerevisiae has been extensively studied, both genetically and by molecular biology; here we report cytological observations of meiotic chromosomal events in this organism. RESULTS: Using fluorescence microscopy, we have examined the behaviour of chromosomes and microtubules during meiosis in S. cerevisiae. We first observed the dynamic behaviour of nuclei in living cells using jellyfish green fluorescent protein (GFP) fused with nucleoplasmin, a Xenopus oocyte nuclear protein. The characterization of nuclear movement in living cells was extended by an analysis of chromosomes and microtubules in fixed specimens. In addition, the nuclear localization of centromeres and telomeres was determined by indirect immunofluorescence microscopy in synchronous populations of meiotic cells. While telomeres remain in clusters of 5-8 throughout meiosis, centromeres change their nuclear localization dramatically during the progression of meiosis: centromeres are first clustered at a single site near the spindle-pole body before the induction of meiosis, and become scattered during the meiotic prophase. CONCLUSIONS: Our observations have demonstrated that nuclear and cytoskeletal reorganization take place with meiosis in S. cerevisiae. In particular, the distinct relocalization of centromeres during meiosis indicates a considerable movement of chromosomes within the meiotic prophase nucleus.
Authors:
A Hayashi; H Ogawa; K Kohno; S M Gasser; Y Hiraoka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genes to cells : devoted to molecular & cellular mechanisms     Volume:  3     ISSN:  1356-9597     ISO Abbreviation:  Genes Cells     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-12-21     Completed Date:  1998-12-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9607379     Medline TA:  Genes Cells     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  587-601     Citation Subset:  IM    
Affiliation:
Kansai Advanced Research Center, Communications Research Laboratory, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe, Japan.
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleus / genetics
Centromere / genetics*
Chromosomes, Fungal*
Green Fluorescent Proteins
Luminescent Proteins / genetics,  metabolism
Meiosis*
Microscopy, Fluorescence
Nuclear Proteins / genetics,  metabolism
Nucleoplasmins
Phosphoproteins / genetics,  metabolism
Prophase
Recombinant Proteins / genetics,  metabolism
Saccharomyces cerevisiae / genetics*
Telomere / genetics*
Chemical
Reg. No./Substance:
0/Luminescent Proteins; 0/Nuclear Proteins; 0/Nucleoplasmins; 0/Phosphoproteins; 0/Recombinant Proteins; 147336-22-9/Green Fluorescent Proteins

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