Document Detail


Meiotic behavior and linkage relationships in the secondarily homothallic fungus Agaricus bisporus.
MedLine Citation:
PMID:  8094696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study followed the transmission of 64 segregating genetic markers to 52 haploid offspring, obtained from both homokaryotic and heterokaryotic meiospores, of a cross (AG 93b) of Agaricus bisporus, the commonly cultivated "button mushroom." The electrophoretic karyotypes of the AG 93b component nuclei were determined concurrently (n = 13). Eleven distinct linkage groups were identified by two-point analysis. DNA-DNA hybridization showed that nine of these corresponded to unique chromosome-sized DNAs. Two other chromosomal DNAs were marked with nonsegregating markers, including the rDNA repeat. Two remaining chromosomes remained unmarked but hybridized to repeated-sequence probes. Cross 93b had an essentially conventional meiosis in which both independent assortment and joint segregation of markers occurred, but in which crossing over was infrequent over much of the mapped genome. The 48 homokaryotic spore-offspring had overall crossover frequencies that were similar to, but possibly slightly less than, those of three homokaryon constituents of heterokaryotic spore-offspring. These daa provide support for our earlier cytogenetic model of sporogenesis in A. bisporus, that explains why heterokaryotic spore-offspring usually appear to exhibit no recombination. No evidence favoring an alternative, mitotic model of sporogenesis was found. The resulting genetic map appears to survey the genome extensively and for the first time permits localization of loci determining economically important traits in this fungal crop species. Large differences in the vigor of homokaryotic offspring were correlated with the inheritance of certain chromosome segments and were also often associated with significant departures from Mendelian segregation ratios.
Authors:
R W Kerrigan; J C Royer; L M Baller; Y Kohli; P A Horgen; J B Anderson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genetics     Volume:  133     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-03-23     Completed Date:  1993-03-23     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  225-36     Citation Subset:  IM    
Affiliation:
Department of Botany, Erindale College, University of Toronto, Mississauga, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Agaricus / genetics*
Base Sequence
Chromosome Mapping
Chromosomes, Fungal
DNA, Fungal / genetics
Genes, Fungal
Genetic Markers
Karyotyping
Linkage (Genetics)
Meiosis
Molecular Sequence Data
Polymorphism, Restriction Fragment Length
Recombination, Genetic
Chemical
Reg. No./Substance:
0/DNA, Fungal; 0/Genetic Markers
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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