Document Detail

Meiotic cytogenetics in Coprinus cinereus.
MedLine Citation:
PMID:  19685322     Owner:  NLM     Status:  MEDLINE    
The basidiomycete fungus Coprinus cinereus has naturally synchronous meiosis and is amenable to analysis using an array of well-developed genetic and molecular tools. In this chapter, we explain in detail the two methods most commonly employed for C. cinereus, staining of intact gill segments and chromosome spreads, with an example of the application of each. We describe iron-hematoxylin staining of intact gill segments for the brightfield examination of meiotic progression, and the use of surface spreads and fluorescence in situ hybridization (FISH) to investigate meiotic chromosome pairing. Gill segments can alternatively be stained with DAPI for the determination of meiotic stage, or propidium iodide for the quantitation of nuclear DNA content, and the chromosome fixation and spreading techniques used for FISH are also suitable for immunolocalization studies of chromosomal proteins.
Miriam E Zolan; Patricia J Pukkila
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Review    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  558     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2009  
Date Detail:
Created Date:  2009-08-17     Completed Date:  2009-10-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  115-27     Citation Subset:  IM    
Department of Biology, Indiana University, Bloomington, IN, USA.
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MeSH Terms
Chromosome Pairing / physiology
Chromosomes, Fungal / genetics,  physiology
Coprinus / cytology*,  genetics*
Cytogenetics / methods*
Meiosis / physiology*
Grant Support

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