Document Detail


Megapixel digital PCR.
MedLine Citation:
PMID:  21725299     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We present a microfluidic 'megapixel' digital PCR device that uses surface tension-based sample partitioning and dehydration control to enable high-fidelity single DNA molecule amplification in 1,000,000 reactors of picoliter volume with densities up to 440,000 reactors cm(-2). This device achieves a dynamic range of 10(7), single-nucleotide-variant detection below one copy per 100,000 wild-type sequences and the discrimination of a 1% difference in chromosome copy number.
Authors:
Kevin A Heyries; Carolina Tropini; Michael Vaninsberghe; Callum Doolin; Oleh I Petriv; Anupam Singhal; Kaston Leung; Curtis B Hughesman; Carl L Hansen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-03
Journal Detail:
Title:  Nature methods     Volume:  -     ISSN:  1548-7105     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101215604     Medline TA:  Nat Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1] Centre for High-Throughput Biology, University of British Columbia, Vancouver, British Columbia, Canada. [2] Department of Physics and Astronomy, University of British Columbia, Vancouver, British Columbia, Canada.
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