Document Detail

Megakaryocyte thromboxane production induced by platelet stimuli during in vitro culture.
MedLine Citation:
PMID:  3918879     Owner:  NLM     Status:  MEDLINE    
Megakaryocytes isolated from guinea pigs produced thromboxane (assayed by radioimmunoassay as thromboxane B2) in response to the platelet aggregatory stimuli arachidonic acid, thrombin, and the calcium ionophore A23187. The relative responses to these stimuli were similar in megakaryocytes and platelets from the same animals. When the megakaryocytes were maintained in short-term in vitro culture, all three stimuli still elicited thromboxane production. Following overnight in vitro culture, thromboxane production in response to thrombin decreased, overall, to under 60% of control values, increased approximately threefold in response to A23187, but did not show any alteration in response to arachidonic acid. Requirements for calcium were virtually unchanged. These results demonstrate that megakaryocytes contain all of the pathways needed for arachidonic acid mobilization from phospholipids in response to thrombin or A23187 and conversion of that arachidonate to thromboxane. These pathways are retained by the cells in short-term in vitro culture.
R W Walenga; J L Miller; M J Stuart
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental hematology     Volume:  13     ISSN:  0301-472X     ISO Abbreviation:  Exp. Hematol.     Publication Date:  1985 Feb 
Date Detail:
Created Date:  1985-03-27     Completed Date:  1985-03-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0402313     Medline TA:  Exp Hematol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  87-91     Citation Subset:  IM    
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MeSH Terms
Arachidonic Acids / pharmacology
Blood Platelets / metabolism
Calcimycin / pharmacology
Calcium / pharmacology
Cells, Cultured
Guinea Pigs
Megakaryocytes / metabolism*
Platelet Aggregation / drug effects
Thrombin / pharmacology
Thromboxane B2 / biosynthesis*
Thromboxanes / biosynthesis*
Grant Support
Reg. No./Substance:
0/Arachidonic Acids; 0/Thromboxanes; 52665-69-7/Calcimycin; 54397-85-2/Thromboxane B2; 7440-70-2/Calcium; EC

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