| Medium- to large-sized xylo-oligosaccharides are responsible for xylanase induction in Prevotella bryantii B14. | |
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MedLine Citation:
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PMID: 16339957 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Experiments were done to define the nature of the xylan-derived induction signal for xylanase activity, and evaluate which xylanase genes among the three known ones (xynA, xynB and xynC) are induced by the presence of xylan in Prevotella bryantii B(1)4. During the later stages of exponential growth on glucose, addition of 0.05 % water-soluble xylan (WS-X) stimulated xylanase formation within 30 min. Xylose, xylobiose, xylotriose, xylotetraose, xylopentaose, arabinose and glucuronic acid all failed to induce the xylanase activity. An acid-ethanol-soluble fraction of WS-X (approximate degree of polymerization 30) enhanced the activity significantly, whereas the acid-ethanol-insoluble fraction had no effect, unless first digested by the cloned P. bryantii XynC xylanase. These results indicate that medium- to large-sized xylo-oligosaccharides are responsible for induction. The transcription of all three known xylanase genes from P. bryantii was upregulated coordinately by addition of WS-X. There have been relatively few investigations into the regulation of xylanase activity in bacteria, and it appears to be unique that medium- to large-sized xylo-oligosaccharides are responsible for induction. |
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Authors:
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Kohji Miyazaki; Tatsuaki Hirase; Yoichi Kojima; Harry James Flint |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Microbiology (Reading, England) Volume: 151 ISSN: 1350-0872 ISO Abbreviation: Microbiology (Reading, Engl.) Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2005-12-12 Completed Date: 2006-02-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9430468 Medline TA: Microbiology Country: England |
Other Details:
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Languages: eng Pagination: 4121-5 Citation Subset: IM |
Affiliation:
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Laboratory of Animal Science, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan. miyazaki@kpu.ac.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Enzyme Induction
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drug effects Gene Expression Regulation, Bacterial / drug effects* Genes, Bacterial Oligosaccharides / pharmacology* Prevotella / enzymology*, genetics Xylan Endo-1,3-beta-Xylosidase / biosynthesis, genetics Xylans / metabolism Xylosidases / biosynthesis*, genetics |
| Chemical | |
Reg. No./Substance:
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0/Oligosaccharides; 0/Xylans; EC 3.2.1.-/Xylosidases; EC 3.2.1.32/Xylan Endo-1,3-beta-Xylosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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