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Medium pH, carbon and nitrogen concentration modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production.
MedLine Citation:
PMID:  21324053     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims:  To study phosphate solubilization in Penicillium purpurogenum as function of medium pH, and carbon and nitrogen concentration. Methods and Results:  Tricalcium phosphate (CP) solubilization efficiency of Penicillium purpurogenum, was evaluated at acid or alkaline pH using different C and N sources. Glucose- and (NH(4) )(2) SO(4) -based media showed the highest P-solubilization values followed by fructose. P. purpurogenum solubilizing ability was higher in cultures grown at pH 6.5 than cultures at pH 8.5. Organic acids were detected in both alkaline and neutral media, but the relative percentages of each organic acid differed. Highest P release coincided with the highest organic acids production peak, especially gluconic acid. When P. purpurogenum grew in alkaline media the organic acids nature and concentration changed at different N and C concentrations. A factorial categorical experimental design showed that the highest P-solubilizing activity, coinciding with the highest organic acids production, corresponded to the highest C concentration and lowest N concentration. Conclusions:  The results described in the present study show that medium pH and carbon and nitrogen concentration modulate the P solubilization efficiency of P. purpurogenum through the production of organic acids, and particularly that of gluconic acid. In the P solubilization optimization studies, glucose and (NH(4) )(2) SO(4) as C and N sources allowed a higher solubilization efficiency at high pH. Significance and Impact of the Study:  This organism is a potentially proficient soil inoculant, especially in P-poor alkaline soils where other P-solubilizers fail to release soluble P. Further work is necessary to elucidate if these results can be extrapolated to natural soil ecosystems, where different pH values are present. P. purpurogenum could be used to develop a bioprocess for the manufacture of phosphatic fertilizer with phosphate calcium minerals.
Authors:
J M Scervino; V L Papinutti; M S Godoy; M A Rodriguez; I Della Monica; M Recchi; M J Pettinari; A M Godeas
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-16
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 The Society for Applied Microbiology.
Affiliation:
Dpto Biodiversidad y Biología Experimental, FCEyN, Universidad de Buenos Aires, Pabellón II, 4P Ciudad Universitaria, (1428) Bs As, Argentina.
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