| Medium optimization for ethanol production with Clostridium autoethanogenum with carbon monoxide as sole carbon source. | |
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MedLine Citation:
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PMID: 20619639 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plackett-Burman and central composite designs were applied to optimize the medium for ethanol production by Clostridium autoethanogenum with CO as sole carbon source, and a medium containing (g/L): NaCl 1.0, KH(2)PO(4) 0.1, CaCl(2) 0.02, yeast extract 0.15, MgSO(4) 0.116, NH(4)Cl 1.694 and pH 4.74 was found optimal. The optimum ethanol yields predicted by response surface methodology (RSM) and an artificial neural network-genetic algorithm (ANN-GA) were 247.48 and 261.48mg/L, respectively. These values are similar to those obtained experimentally under the optimal conditions suggested by the statistical methods (254.26 and 259.64mg/L). The fitness of the ANN-GA model was higher than that of the RSM model. The yields obtained substantially exceed those previously reported (60-70mg/L) with this organism. |
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Authors:
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Ying Guo; Jingliang Xu; Yu Zhang; Huijuan Xu; Zhenhong Yuan; Dong Li |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-08 |
Journal Detail:
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Title: Bioresource technology Volume: 101 ISSN: 1873-2976 ISO Abbreviation: Bioresour. Technol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-08-02 Completed Date: 2011-01-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9889523 Medline TA: Bioresour Technol Country: England |
Other Details:
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Languages: eng Pagination: 8784-9 Citation Subset: IM |
Affiliation:
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Key Laboratory of Renewable Energy and Gas Hydrate, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms* Bioreactors / microbiology* Carbon Monoxide / metabolism* Cell Culture Techniques / methods Clostridium acetobutylicum / growth & development, metabolism* Computer Simulation Culture Media / chemistry, metabolism Ethanol / metabolism* Models, Biological* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 630-08-0/Carbon Monoxide; 64-17-5/Ethanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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