| Mediator phosphorylation prevents stress response transcription during non-stress conditions. | |
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MedLine Citation:
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PMID: 23135281 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The multiprotein complex Mediator is a coactivator of RNA polymerase (Pol) II transcription that is required for the regulated expression of protein-coding genes. Mediator serves as an end point of signaling pathways and regulates Pol II transcription, but the mechanisms it uses are not well understood. Here, we used mass spectrometry and dynamic transcriptome analysis to investigate a functional role of Mediator phosphorylation in gene expression. Affinity purification and mass spectrometry revealed that Mediator from the yeast Saccharomyces cerevisiae is phosphorylated at multiple sites of 17 of its 25 subunits. Mediator phosphorylation levels change upon an external stimulus set by exposure of cells to high salt concentrations. Phosphorylated sites in the Mediator tail subunit Med15 are required for suppression of stress-induced changes in gene expression under non-stress conditions. Thus dynamic and differential Mediator phosphorylation contributes to gene regulation in eukaryotic cells. |
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Authors:
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Christian Miller; Ivan Matic; Kerstin C Maier; Björn Schwalb; Susanne Roether; Katja Strässer; Achim Tresch; Matthias Mann; Patrick Cramer |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-11-07 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 287 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-31 Completed Date: 2013-03-25 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 44017-26 Citation Subset: IM |
Affiliation:
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Gene Center Munich and Department of Biochemistry, Ludwig-Maximilians-Universität München, Feodor-Lynen-Strasse 25, 81377 Munich, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Motifs Gene Expression Regulation, Fungal* Mediator Complex / chemistry, genetics, metabolism* Phosphorylation RNA Polymerase II / genetics, metabolism Saccharomyces cerevisiae / chemistry, genetics*, metabolism* Saccharomyces cerevisiae Proteins / chemistry, genetics, metabolism* Transcription, Genetic* |
| Chemical | |
Reg. No./Substance:
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0/GAL11 protein, S cerevisiae; 0/Mediator Complex; 0/Saccharomyces cerevisiae Proteins; EC 2.7.7.-/RNA Polymerase II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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