Document Detail

Mediator phosphorylation prevents stress response transcription during non-stress conditions.
MedLine Citation:
PMID:  23135281     Owner:  NLM     Status:  MEDLINE    
The multiprotein complex Mediator is a coactivator of RNA polymerase (Pol) II transcription that is required for the regulated expression of protein-coding genes. Mediator serves as an end point of signaling pathways and regulates Pol II transcription, but the mechanisms it uses are not well understood. Here, we used mass spectrometry and dynamic transcriptome analysis to investigate a functional role of Mediator phosphorylation in gene expression. Affinity purification and mass spectrometry revealed that Mediator from the yeast Saccharomyces cerevisiae is phosphorylated at multiple sites of 17 of its 25 subunits. Mediator phosphorylation levels change upon an external stimulus set by exposure of cells to high salt concentrations. Phosphorylated sites in the Mediator tail subunit Med15 are required for suppression of stress-induced changes in gene expression under non-stress conditions. Thus dynamic and differential Mediator phosphorylation contributes to gene regulation in eukaryotic cells.
Christian Miller; Ivan Matic; Kerstin C Maier; Björn Schwalb; Susanne Roether; Katja Strässer; Achim Tresch; Matthias Mann; Patrick Cramer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-07
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  287     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-31     Completed Date:  2013-03-25     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  44017-26     Citation Subset:  IM    
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MeSH Terms
Amino Acid Motifs
Gene Expression Regulation, Fungal*
Mediator Complex / chemistry,  genetics,  metabolism*
RNA Polymerase II / genetics,  metabolism
Saccharomyces cerevisiae / chemistry,  genetics*,  metabolism*
Saccharomyces cerevisiae Proteins / chemistry,  genetics,  metabolism*
Transcription, Genetic*
Reg. No./Substance:
0/GAL11 protein, S cerevisiae; 0/Mediator Complex; 0/Saccharomyces cerevisiae Proteins; EC 2.7.7.-/RNA Polymerase II

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