Document Detail


The mediator complex subunit PFT1 interferes with COP1 and HY5 in the regulation of Arabidopsis light signaling.
MedLine Citation:
PMID:  22760208     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Arabidopsis (Arabidopsis thaliana) mutants hypersensitive to far-red light were isolated under a light program of alternating red and far-red light pulses and were named eid (for empfindlicher im dunkelroten Licht). The dominant eid3 mutant carries a missense mutation in a conserved domain of PHYTOCHROME AND FLOWERING TIME1 (PFT1), an important component of the plant mediator coactivator complex, which links promoter-bound transcriptional regulators to RNA polymerase II complexes. Epistatic analyses were performed to obtain information about the coaction between the mutated PFT1(eid3) and positively and negatively acting components of light signaling cascades. The data presented here provide clear evidence that the mutation mainly enhances light sensitivity downstream of phytochrome A (phyA) and modulates phyB function. Our results demonstrate that the Mediator component cooperates with CONSTITUTIVE PHOTORMORPHOGENIC1 in the regulation of light responses and that the hypersensitive phenotype strictly depends on the presence of the ELONGATED HYPOCOTYL5 transcription factor, an important positive regulator of light-dependent gene expression. Expression profile analyses revealed that PFT1(eid3) alters the transcript accumulation of light-regulated genes even in darkness. Our data further indicate that PFT1 regulates the floral transition downstream of phyA. The PFT1 missense mutation seems to create a constitutively active transcription factor by mimicking an early step in light signaling.
Authors:
Cornelia Klose; Claudia Büche; Aurora Piñas Fernandez; Eberhard Schäfer; Eva Zwick; Thomas Kretsch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-03
Journal Detail:
Title:  Plant physiology     Volume:  160     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-04     Completed Date:  2013-01-10     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  289-307     Citation Subset:  IM    
Affiliation:
Albert-Ludwigs-Universität Freiburg, Faculty of Biology, Institut für Biologie 2, Botanik, 79104 Freiburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Anthocyanins / metabolism
Arabidopsis / genetics,  growth & development,  metabolism,  radiation effects*
Arabidopsis Proteins / genetics,  metabolism*
Basic-Leucine Zipper Transcription Factors / genetics,  metabolism*
Chromosome Mapping
Conserved Sequence
Epistasis, Genetic
Flowers / growth & development
Gene Expression Regulation, Plant
Genes, Plant
Light*
Mutation, Missense
Nuclear Proteins / genetics,  metabolism*
Phenotype
Photoperiod
Phytochrome A / genetics,  metabolism
Phytochrome B / genetics,  metabolism
Protein Structure, Tertiary
Proteolysis
Signal Transduction
Transcription, Genetic
Ubiquitin-Protein Ligases / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Anthocyanins; 0/Arabidopsis Proteins; 0/Basic-Leucine Zipper Transcription Factors; 0/HY5 protein, Arabidopsis; 0/Nuclear Proteins; 0/PFT1 protein, Arabidopsis; 0/PHYA protein, Arabidopsis; 0/PHYB protein, Arabidopsis; 0/Phytochrome A; 136250-22-1/Phytochrome B; EC 6.3.2.19/COP1 protein, Arabidopsis; EC 6.3.2.19/Ubiquitin-Protein Ligases
Comments/Corrections

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