Document Detail

Medial prefrontal cortex neuronal activation and synaptic alterations after stress-induced reinstatement of palatable food seeking: a study using c-fos-GFP transgenic female rats.
MedLine Citation:
PMID:  22723688     Owner:  NLM     Status:  MEDLINE    
Relapse to maladaptive eating habits during dieting is often provoked by stress and there is evidence for a role of ovarian hormones in stress responses and feeding. We studied the role of these hormones in stress-induced reinstatement of food seeking and medial prefrontal cortex (mPFC) neuronal activation in c-fos-GFP transgenic female rats, which express GFP in strongly activated neurons. Food-restricted ovariectomized or sham-operated c-fos-GFP rats were trained to lever-press for palatable food pellets. Subsequently, lever-pressing was extinguished and reinstatement of food seeking and mPFC neuronal activation was assessed after injections of the pharmacological stressor yohimbine (0.5-2 mg/kg) or pellet priming (1-4 noncontingent pellets). Estrous cycle effects on reinstatement were also assessed in wild-type rats. Yohimbine- and pellet-priming-induced reinstatement was associated with Fos and GFP induction in mPFC; both reinstatement and neuronal activation were minimally affected by ovarian hormones in both c-fos-GFP and wild-type rats. c-fos-GFP transgenic rats were then used to assess glutamatergic synaptic alterations within activated GFP-positive and nonactivated GFP-negative mPFC neurons following yohimbine-induced reinstatement of food seeking. This reinstatement was associated with reduced AMPA receptor/NMDA receptor current ratios and increased paired-pulse facilitation in activated GFP-positive but not GFP-negative neurons. While ovarian hormones do not appear to play a role in stress-induced relapse of food seeking in our rat model, this reinstatement was associated with unique synaptic alterations in strongly activated mPFC neurons. Our paper introduces the c-fos-GFP transgenic rat as a new tool to study unique synaptic changes in activated neurons during behavior.
Carlo Cifani; Eisuke Koya; Brittany M Navarre; Donna J Calu; Michael H Baumann; Nathan J Marchant; Qing-Rong Liu; Thi Khuc; James Pickel; Carl R Lupica; Yavin Shaham; Bruce T Hope
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  32     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-22     Completed Date:  2012-08-30     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8480-90     Citation Subset:  IM    
Intramural Research Program, NIDA/NIH, Baltimore, Maryland 21224, USA.
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MeSH Terms
Corticosterone / blood
Electrophysiological Phenomena
Estrous Cycle / physiology
Excitatory Postsynaptic Potentials / physiology
Feeding Behavior / physiology*
Genes, fos / genetics,  physiology*
Green Fluorescent Proteins / genetics
Neurons / physiology*
Patch-Clamp Techniques
Prefrontal Cortex / physiology*
Pyramidal Cells / drug effects
Rats, Long-Evans
Rats, Transgenic
Stress, Psychological / psychology*
Sympatholytics / pharmacology
Synapses / physiology*
Yohimbine / pharmacology
Grant Support
Reg. No./Substance:
0/Sympatholytics; 146-48-5/Yohimbine; 147336-22-9/Green Fluorescent Proteins; 50-22-6/Corticosterone

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