| Medial prefrontal cortex neuronal activation and synaptic alterations after stress-induced reinstatement of palatable food seeking: a study using c-fos-GFP transgenic female rats. | |
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MedLine Citation:
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PMID: 22723688 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Relapse to maladaptive eating habits during dieting is often provoked by stress and there is evidence for a role of ovarian hormones in stress responses and feeding. We studied the role of these hormones in stress-induced reinstatement of food seeking and medial prefrontal cortex (mPFC) neuronal activation in c-fos-GFP transgenic female rats, which express GFP in strongly activated neurons. Food-restricted ovariectomized or sham-operated c-fos-GFP rats were trained to lever-press for palatable food pellets. Subsequently, lever-pressing was extinguished and reinstatement of food seeking and mPFC neuronal activation was assessed after injections of the pharmacological stressor yohimbine (0.5-2 mg/kg) or pellet priming (1-4 noncontingent pellets). Estrous cycle effects on reinstatement were also assessed in wild-type rats. Yohimbine- and pellet-priming-induced reinstatement was associated with Fos and GFP induction in mPFC; both reinstatement and neuronal activation were minimally affected by ovarian hormones in both c-fos-GFP and wild-type rats. c-fos-GFP transgenic rats were then used to assess glutamatergic synaptic alterations within activated GFP-positive and nonactivated GFP-negative mPFC neurons following yohimbine-induced reinstatement of food seeking. This reinstatement was associated with reduced AMPA receptor/NMDA receptor current ratios and increased paired-pulse facilitation in activated GFP-positive but not GFP-negative neurons. While ovarian hormones do not appear to play a role in stress-induced relapse of food seeking in our rat model, this reinstatement was associated with unique synaptic alterations in strongly activated mPFC neurons. Our paper introduces the c-fos-GFP transgenic rat as a new tool to study unique synaptic changes in activated neurons during behavior. |
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Authors:
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Carlo Cifani; Eisuke Koya; Brittany M Navarre; Donna J Calu; Michael H Baumann; Nathan J Marchant; Qing-Rong Liu; Thi Khuc; James Pickel; Carl R Lupica; Yavin Shaham; Bruce T Hope |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Intramural |
Journal Detail:
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Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 32 ISSN: 1529-2401 ISO Abbreviation: J. Neurosci. Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-06-22 Completed Date: 2012-08-30 Revised Date: 2013-04-01 |
Medline Journal Info:
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Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: 8480-90 Citation Subset: IM |
Affiliation:
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Intramural Research Program, NIDA/NIH, Baltimore, Maryland 21224, USA. |
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Corticosterone / blood Electrophysiological Phenomena Estrous Cycle / physiology Excitatory Postsynaptic Potentials / physiology Feeding Behavior / physiology* Female Genes, fos / genetics, physiology* Green Fluorescent Proteins / genetics Immunohistochemistry Neurons / physiology* Ovariectomy Patch-Clamp Techniques Prefrontal Cortex / physiology* Pyramidal Cells / drug effects Rats Rats, Long-Evans Rats, Transgenic Stress, Psychological / psychology* Sympatholytics / pharmacology Synapses / physiology* Yohimbine / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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ZIA DA000467-08/DA/NIDA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Sympatholytics; 146-48-5/Yohimbine; 147336-22-9/Green Fluorescent Proteins; 50-22-6/Corticosterone |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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