Document Detail


Mechanosensitivity of human osteosarcoma cells and phospholipase C beta2 expression.
MedLine Citation:
PMID:  15939402     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bone adapts to mechanical load by osteosynthesis, suggesting that osteoblasts might respond to mechanical stimuli. We therefore investigated cell proliferation and phospholipase C (PLC) expression in osteoblasts. One Hertz uniaxial stretching at 4000 microstrains significantly increased the proliferation rates of human osteoblast-like osteosarcoma cell line MG-63 and primary human osteoblasts. However, U-2/OS, SaOS-2, OST, and MNNG/HOS cells showed no significant changes in proliferation rate. We investigated the expression pattern of different isoforms of PLC in these cell lines. We were able to detect PLC beta1, beta3, gamma1, gamma2, and delta1 in all cells, but PLC beta2 was only detectable in the mechanosensitive cells. We therefore investigated the possible role of PLC beta2 in mechanotransduction. Inducible antisense expression for 24h inhibited the translation of PLC beta1 in U-2/OS cells by 35% and PLC beta2 in MG-63 by 29%. Fluid shear flow experiments with MG-63 lacking PLC beta2 revealed a significantly higher level of cells losing attachment to coverslips and a significantly lower number of cells increasing intracellular free calcium.
Authors:
M Hoberg; H-H Gratz; M Noll; D B Jones
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  333     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-06-16     Completed Date:  2005-08-15     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  142-9     Citation Subset:  IM    
Affiliation:
Department of Orthopaedics, University of Tuebingen, Germany. Maik.Hoberg@med.uni-tuebingen.de
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MeSH Terms
Descriptor/Qualifier:
Cell Adhesion
Cell Line
Cell Proliferation
Cell Survival
Humans
Isoenzymes / metabolism*
Mechanotransduction, Cellular*
Osteoblasts / physiology*
Osteosarcoma / physiopathology*
Phospholipase C beta
Physical Stimulation / methods*
Type C Phospholipases / metabolism*
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 3.1.4.-/Type C Phospholipases; EC 3.1.4.11/PLCB1 protein, human; EC 3.1.4.11/PLCB2 protein, human; EC 3.1.4.11/PLCB3 protein, human; EC 3.1.4.11/Phospholipase C beta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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