| Mechanosensitive release of parathyroid hormone-related peptide from coronary endothelial cells. | |
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MedLine Citation:
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PMID: 12234801 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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10.1152/ajpheart.00925. 2001.-Parathyroid hormone-related peptide (PTHrP) is expressed throughout the cardiovascular system and is able to dilate vessels. This study investigated whether mechanical forces generated by changes in regional perfusion influence PTHrP release from the coronary vascular bed. Experiments were performed in vitro on saline-perfused rat hearts or isolated coronary endothelial cells exposed to cyclic strain and in vivo in anesthetized pigs. In vitro, PTHrP release from saline-perfused rat hearts was strongly correlated with coronary flow (r = 0.84). Increasing coronary flow from 5 to 10 ml/min increased PTHrP release from 442 +/- 42 to 1,563 +/- 167 pg/min. Increasing the viscosity of the perfusate did not change basal PTHrP release. Increasing flow without a concomitant increase in pressure did not lead to an increase in release rate, but reduction in pressure under flow-constant conditions reduced PTHrP release rate. Cyclic strain induced a strain-dependent release of PTHrP from endothelial cells that was inhibited by the addition of a calcium-chelating agent. In vivo, there was a net release of PTHrP in the coronary circulation and decreases in coronary flow and pressure decreased the PTHrP release rate. Bradykinin in the presence of constant pressure increased PTHrP release, probably by increasing the intracellular calcium concentration in coronary endothelial cells. In summary, mechanical forces evoked by blood flow can trigger a constant PTHrP release. |
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Authors:
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Heike Degenhardt; Johanna Jansen; Rainer Schulz; Daniel Sedding; Ruediger Braun-Dullaeus; Klaus-Dieter Schlüter |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 283 ISSN: 0363-6135 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2002 Oct |
Date Detail:
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Created Date: 2002-09-17 Completed Date: 2002-10-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
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Languages: eng Pagination: H1489-96 Citation Subset: IM; S |
Affiliation:
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Physiologisches Institut, Justus-Liebig-Universität, D-35392 Giessen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arginine / pharmacology Blood Pressure / physiology Bradykinin / pharmacology Cardiotonic Agents / pharmacology Coronary Vessels / cytology, metabolism* Endothelium, Vascular / cytology, metabolism* Enzyme Inhibitors / pharmacology Male Nitric Oxide / metabolism Nitric Oxide Donors / pharmacology Nitroarginine / pharmacology Nitrogen Oxides Parathyroid Hormone-Related Protein Perfusion Phenylephrine / pharmacology Proteins / metabolism* Sodium Chloride Spermine / analogs & derivatives*, pharmacology Stress, Mechanical Swine, Miniature Vascular Resistance / drug effects, physiology |
| Chemical | |
Reg. No./Substance:
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0/Cardiotonic Agents; 0/Enzyme Inhibitors; 0/Nitric Oxide Donors; 0/Nitrogen Oxides; 0/Parathyroid Hormone-Related Protein; 0/Proteins; 10102-43-9/Nitric Oxide; 136587-13-8/spermine nitric oxide complex; 2149-70-4/Nitroarginine; 58-82-2/Bradykinin; 59-42-7/Phenylephrine; 71-44-3/Spermine; 74-79-3/Arginine; 7647-14-5/Sodium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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