Document Detail


Mechanosensitive hair cell-like cells from embryonic and induced pluripotent stem cells.
MedLine Citation:
PMID:  20478259     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mechanosensitive sensory hair cells are the linchpin of our senses of hearing and balance. The inability of the mammalian inner ear to regenerate lost hair cells is the major reason for the permanence of hearing loss and certain balance disorders. Here, we present a stepwise guidance protocol starting with mouse embryonic stem and induced pluripotent stem cells, which were directed toward becoming ectoderm capable of responding to otic-inducing growth factors. The resulting otic progenitor cells were subjected to varying differentiation conditions, one of which promoted the organization of the cells into epithelial clusters displaying hair cell-like cells with stereociliary bundles. Bundle-bearing cells in these clusters responded to mechanical stimulation with currents that were reminiscent of immature hair cell transduction currents.
Authors:
Kazuo Oshima; Kunyoo Shin; Marc Diensthuber; Anthony W Peng; Anthony J Ricci; Stefan Heller
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  141     ISSN:  1097-4172     ISO Abbreviation:  Cell     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-18     Completed Date:  2010-05-20     Revised Date:  2014-09-13    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  704-16     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques
Cell Differentiation
Embryonic Stem Cells / cytology*
Green Fluorescent Proteins / genetics,  metabolism
Hair Cells, Auditory / cytology*,  physiology,  ultrastructure
Hair Cells, Vestibular / cytology*,  physiology,  ultrastructure
Induced Pluripotent Stem Cells / cytology*
Mechanotransduction, Cellular
Mice
Grant Support
ID/Acronym/Agency:
DC006167/DC/NIDCD NIH HHS; P30 DC010363/DC/NIDCD NIH HHS; P30 DC010363-01/DC/NIDCD NIH HHS; P30 DC010363-017735/DC/NIDCD NIH HHS; P30 DC010363-017736/DC/NIDCD NIH HHS; P30DC010363/DC/NIDCD NIH HHS; R01 DC003896/DC/NIDCD NIH HHS; R01 DC006167/DC/NIDCD NIH HHS; R01 DC006167-01/DC/NIDCD NIH HHS; R01 DC006167-02/DC/NIDCD NIH HHS; R01 DC006167-03/DC/NIDCD NIH HHS; R01 DC006167-04/DC/NIDCD NIH HHS; R01 DC006167-05/DC/NIDCD NIH HHS; R01 DC006167-06/DC/NIDCD NIH HHS; R01 DC006167-07A2/DC/NIDCD NIH HHS; R01 DC006167-07A2S1/DC/NIDCD NIH HHS; R01 DC006167-09/DC/NIDCD NIH HHS; R21 DC010042-01/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

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