Document Detail

Mechanistic studies of polyene enhancement of interferon production by polyriboinosinic-polyribocytidylic acid.
MedLine Citation:
PMID:  206199     Owner:  NLM     Status:  MEDLINE    
The production of interferon by polyriboinosinic-polyribocytidylic acid [poly(I) . poly(C)] and poly(I) . poly(C)-diethylaminoethyl-dextran in L929 cells was enhanced from 10 to 100 times by polyene macrolides, including amphotericin B (AmB), AmB methyl ester, nystatin, and filipin. AmB and its water-soluble methyl ester were the most effective; retinol, a nonmacrolide polyene, was ineffective. Interferon induction by Newcastle disease virus was not enhanced by AmB. The kinetics of interferon production were not markedly altered by AmB. Polyenes and poly(I) . poly(C)-diethylaminoethyl-dextran did not need to be present on cells simultaneously to enhance interferon production. Pretreatment with polyenes was as effective as simultaneous addition. Even treatment of washed cells, several hours after removal of poly(I) . poly(C)-diethylaminoethyl-dextran, resulted in enhancement of interferon production. AmB did not appear to form a macromolecular complex with poly(I) . poly(C) in that neither the ultraviolet absorption spectrum nor the melting point of poly(I) . poly(C) was altered by mixing with AmB. Isotopic studies indicated that AmB did not enhance binding of poly(I) . poly(C) to cells. Since the macrolide polyenes have been demonstrated to bind to cell membrane sterols with subsequent alterations in membrane permeability barriers, they may enhance interferon production by increasing cell penetration of poly(I) . poly(C).
E C Borden; B W Booth; P H Leonhardt
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  13     ISSN:  0066-4804     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  1978 Feb 
Date Detail:
Created Date:  1978-06-12     Completed Date:  1978-06-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  159-64     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
Drug Synergism
Interferon Inducers*
Poly I-C / pharmacology*
Polyenes / pharmacology*
Time Factors
Vesicular stomatitis Indiana virus
Reg. No./Substance:
0/Interferon Inducers; 0/Polyenes; 24939-03-5/Poly I-C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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