Document Detail

Mechanistic studies on the photodynamic effect induced by a dicationic fullerene C60 derivative on Escherichia coli and Candida albicans cells.
MedLine Citation:
PMID:  23993859     Owner:  NLM     Status:  In-Data-Review    
The photodynamic mechanism of action induced by N,N-dimethyl-2-(4'-N,N,N-trimethylaminophenyl)fulleropyrrolidinium iodide (DTC60(2+)) was investigated on Candida albicans and Escherichia coli cells. First, photogeneration of superoxide anion radical by DTC60(2+) in the presence of NADH was detected using nitro blue tetrazolium method in reverse micelles. In C. albicans suspensions, 10μM DTC60(2+) was an effective photosensitizer, producing a ∼5log decrease of cell survival when the cultures were irradiated for 30min with visible light. Also, C. albicans cells growth was not detected in the presence of 10μM DTC60(2+) and irradiation. Photodynamic mechanism investigations were compared in both C. albicans and E. coli cells. Studies under anoxic conditions indicated that oxygen was required for the photodynamic inactivation of these microorganisms. The photocytotoxicity induced by DTC60(2+) was similar in D2O than in water cell suspensions. Furthermore, photoinactivation of microbial cells was negligible in the presence of azide ion, while the addition of mannitol produced a photoprotective effect on the cellular survival. These results indicate that DTC60(2+) has potential as agent to the photodynamic inactivation of microbial cells. Also, the photocytotoxicity activity induced by this cationic fullerene derivative can involve the intermediacy of both superoxide anion radical and singlet molecular oxygen.
M Elisa Milanesio; Mariana B Spesia; M Paula Cormick; Edgardo N Durantini
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Publication Detail:
Type:  Journal Article     Date:  2013-03-06
Journal Detail:
Title:  Photodiagnosis and photodynamic therapy     Volume:  10     ISSN:  1873-1597     ISO Abbreviation:  Photodiagnosis Photodyn Ther     Publication Date:  2013 Sep 
Date Detail:
Created Date:  2013-09-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101226123     Medline TA:  Photodiagnosis Photodyn Ther     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  320-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier B.V. All rights reserved.
Departamento de Química, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Agencia Postal Nro 3, X5804BYA Río Cuarto, Córdoba, Argentina.
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